In this paper we prove that the initial value problem associated to the following higher-order Benjamin-Ono equation ∂tv − bH∂ xv + a∂ xv = cv∂xv − d∂x(vH∂xv + H(v∂xv)), where x, t ∈ R, v is a real-valued function, H is the Hilbert transform, a ∈ R, b, c and d are positive constants, is locally well-posed for initial data v(0) = v0 ∈ H(R), s ≥ 2 or v0 ∈ H(R) ∩ L(R; xdx), k ∈ Z+, k ≥ 2.