background: mesenchymal stem cells (mscs) are undifferentiated cells that can differentiate and divide to other cell types. transplantation of these cells to the different organs is used for curing various diseases. objective: the aim of this research was whether mscs transplantation could treat the sterile testes. materials and methods: in this experimental study, donor mscs were isolated from...

ex vivo expansion of human umbilical cord blood cells (hucbc) is explored by several investigators to enhance the repopulating potential of hucbc. the proliferation and expansion of human hematopoietic stem cells (hsc) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding hsc for patient transplantation. using primary human mesenchymal stem cells...

The aim of the present study was to examine the frequencies of cell proliferation and death of granulosa and theca interna layers during development of cystic follicles in order to understand the mechanisms of cystic follicle formation. Paraffin sections of cystic follicles were immunostained with antibodies against proliferating cell nuclear antigen (PCNA) and cleaved caspase-3 in order to obs...

The theca interna is a specialized stromal layer that envelops each growing ovarian follicle. It contains capillaries, fibroblasts, immune cells and the steroidogenic cells that synthesize androgens for conversion to estradiol by the neighboring granulosa cells. During reproductive life only a small number of follicles will grow to a sufficient size to ovulate, whereas the majority of follicles...

objectives: the study of erythropoiesis needs to develop the methods for erythroid progenitor cells (epcs) culture using stem cell potency to differentiate into variety of hematopoietic cells lineages. in this study, we induced differentiation of cord blood stem cells into erythroid progenitor cells in a semisolid culture media. materials and methods: cord blood mononuclear cells were isolate...

neural stem cells (nscs) as a heterogeneous multipotent and self- renewal population are found in different areas in the developing mammalian nervous system, as well as the sub-ventricular zone (svz) and the hippocampus of the adult brain. nscs can give rise to neurons, astrocytes and oligodendrocytes. the aim of this study was to differentiate neural stem cells into noradrenergic–like cells in...

background: mesenchymal stem cells (mscs) are multipotent cells that can be collected from different sources. under specific conditions, mscs can be differentiated to tissue specific cells in vitro. human umbilical cord mesenchymal stem cells (hucmscs) can easily be harvested and cultured in in vitro conditions. production of germ cells from mesenchymal stem cells is a very interesting and prom...

objective(s): electrospun nanofibrous mats of poly(lactic acid) (pla) and nanohydroxyapatite (nano-ha) were prepared and proliferation and differentiation of mesenchymal stem cells on the prepared nanofibers were investigated in this study. materials and methods: pla/nano-ha nanofibers were prepared by electrospinning. the effects of process parameters, such as nano-ha concentration, distance, ...

primary and secondary malignant central nervous system (cns) tumors are devastating invasive tumors able to give rise to many kinds of differentiated tumor cells. glioblastoma multiform (gbm), is the most malignant brain tumor, in which its growth and persistence depend on cancer stem cells with enhanced dna damage repair program that also induces recurrence and resists current chemo- and radio...

The theca interna layer of the ovarian follicle forms during the antral stage of follicle development and lies adjacent to and directly outside the follicular basal lamina. It supplies androgens and communicates with the granulosa cells and the oocyte by extracellular signaling. To better understand developmental changes in the theca interna, we undertook transcriptome profiling of the theca in...