The frequency of inherited variations in thermostability was investigated in a series of seven enzymes in a Japanese population. Among a total of 5930 determinations, nine variants were encountered. In each instance one parent exhibited a similar finding. It is suggested that this procedure should detect a high proportion of the variants of these enzymes characterized by amino acid substitution...

Background: Phytate is an anti-nutritional factor in plants, which catches the most phosphorus contents and some vital minerals. Therefore, Phytase is added mainly as an additive to the monogastric animals’ foods to hydrolyze phytate and increase absorption of phosphorus. Objectives: Y. intermedia phytase is a new phytase with special characteristics such as high specific activity, pH stabilit...

Studies on the irreversible thermoinactivation of beta-amylase from Bacillus megaterium B6 exposed to 60 degrees C revealed that the deactivation mechanism probably results from the oxidation of thiols present at the active site of the enzyme. Several attempts were made to increase its thermostability, which indicated that Mn2+ played a key role in determining thermostability and partially reac...

The thermostability and density of water-salt solutions of DNA, irradiated by non thermal coherent millimeter electromagnetic waves with frequency 64.5 GHz have been investigated using the methods of spectrophotometry and densitometry. It is shown that the thermostability of DNA and density of its solutions are increased, depending on time of irradiation. It is expected that under the influence...

The three-dimensional structure of a thermostable beta-glycosidase (Gly(Tn)) from the thermophilic eubacterium Thermus nonproteolyticus HG102 was determined at a resolution of 2.4 A. The core of the structure adopts the (betaalpha)(8) barrel fold. The sequence alignments and the positions of the two Glu residues in the active center indicate that Gly(Tn) belongs to the glycosyl hydrolases of re...

Abstract The cellulase cocktail of marine Aspergillus niger exhibited salt-tolerant and thermostable properties, which is great potential in industrial application. In order to excavate the single tolerant components from complex cocktail, constitutive homologous expression was employed for direct obtainment endoglucanase ( An EGL). Enzymatic property study revealed that EGL a salt tolerance st...