An ultrasensitive method for the determination of proteins is described that combines an enzyme-linked immunosorbent assay (ELISA) and a thionicotinamide-adenine dinucleotide (thio-NAD) cycling method. A sandwich method using a primary and a secondary antibody for antigens is employed in an ELISA. An androsterone derivative, 3α-hydroxysteroid, is produced by the hydrolysis of 3α-hydroxysteroid ...

Enantiomerically pure 2-C-methyl-D-erythritol 4-phosphate 1 (MEP) is synthesized from 1,2-O-isopropylidene-α-D-xylofuranose via facile benzylation in good yield. Subsequently, 1 is used for enzymatic synthesis of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2 (CDP-ME) using 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase (IspD). The chemoenzymatically synthesized 2 can be used as substrate fo...

recently several 1,3,4-oxadiazole derivatives were identified as potentially active antimycobacterial agents. various 5-aryl-2-thio-1,3,4-oxadiazoles have been reported having good antimycobacterial activity against mycobacterium tuberculosis h37rv (atcc 27294). in this paper we report 3d qsar studies for the 41 molecules of 1,3,4-oxadiazoles by using k-nearest neighbor molecular field analysis...

In the title complex, [Hg(NCS)2(CH4N2S)2], the Hg(II) atom is four-coordinated having an irregular four-coordinate geometry composed of four thione S atoms of two thio-cyanate groups and two thio-urea groups. The S-Hg-S angles are 172.02 (9)° for the trans-thio-cyanate S atoms and 90.14 (5)° for the cis-thio-urea S atoms. The mol-ecular structure is stabilized by an intra-molecular N-H⋯S hydrog...

The title compound, C(13)H(12)N(2)OS, was obtained from the condensation reaction of 2-acetyl-thio-phene and benzohydrazide. In the mol-ecule, the formohydrazide fragment is approximately planar (r.m.s deviation = 0.0146 Å) and the mean plane is oriented at dihedral angles of 24.47 (11) and 28.86 (13)°, respectively, to the phenyl and thio-phene rings. The thio-phene and phenyl rings make a dih...

Abstract Telomerase reverse transcriptase (TERT) is essential for tumor immortality. Since TERT silenced in normal cells, it an attractive therapeutic target and the inhibitor 6-thio-2’-deoxyguanosine (6-thio-dG) clinical trials gliomas. However, there a lag period following inhibition before shrinkage observed. Novel strategies that can be combined with to enhance anti-tumor activity are sorel...

Objective: The aim of the work was histological study a corrective influence sodium 2-((4-amino-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-3-yl) thio) acetate on state rats liver under conditions acute immobilization stress. Material and Method: As an object research used. All substances were injected intragastrically, daily for 5 days in appropriate doses empty stomach from 9.00-10.00. 1 hour af...

Protein-nanoparticle interactions are important in biomedical applications of nanoparticles and for growing biosafety concerns about nanomaterials. In this study, the interactions of four plasma proteins, human serum albumin (HSA), myoglobin (MB), hemoglobin (HB), and trypsin (TRP), with Au and Ag nanoparticles were investigated by FT-IR spectroscopy. The secondary structure of thio-proteins ch...