Analysis of phylogenomic metrics of a recently released draft genome sequence of the halotolerant, acidophile 'Thiobacillus prosperus' DSM 5130 indicates that it is not a member of the genus Thiobacillus within the class Betaproteobacteria as originally proposed. Based on data from 16S rRNA gene phylogeny, and analyses of multiprotein phylogeny and average nucleotide identity (ANI), we show tha...

The complete 5S and 16S ribosomal DNA (rDNA) sequences of the facultatively chemolithotrophic bacterium Thiobacillus cuprinus and results of a comparison of these sequences with homologous sequences from several proteobacterial species supported affiliation of T. cuprinus with the beta 1 subgroup of the Proteobacteria. T. cuprinus, Thiobacillus intermedius, Thiobacillus perometabolis, and Thiob...

The type strain (ATCC 23270) and two other strains of Thiobacillus ferrooxidans were able to grow by hydrogen oxidation, a feature not recognized before. When cultivated on H2, a hydrogenase was induced and the strains were less extremely acidophilic than during growth on sulfidic ores. Cells of T. ferrooxidans grown on H2 and on ferrous iron showed 100% DNA homology. Hydrogen oxidation was not...

Biodesulfurization of coal has been carried out in batch culture of single strain and mixed culture of sulfur oxidizing bacteria. The pure cultures of Thiobacillus thiooxidans and Thiobacillus ferrooxidans utilized inorganic sulfur content of Malaysian coal. The chemolithotroph bacteria were able to grow on coal and metabolize the coal's sulfur content. The batch cultures of coal with 3-5 perce...

Schaeffer, W. I. (Rutgers, The State University, New Brunswick, N.J.), P. E. Holbert, and W. W. Umbreit. Attachment of Thiobacillus thiooxidans to sulfur crystals. J. Bacteriol. 85:137-140. 1963.-Electron micrographs of replicas of sulfur crystals before and after attack by Thiobacillus thiooxidans show that the microorganisms erode the crystal in the area immediately adjacent to the cell. When...

High concentrations of H(2)S in groundwater are commonly removed using Biological Trickling Filter (BTF) that contains high numbers of biofilm immobilized sulfur oxidizing bacteria (mainly Thiobacillus thiooxidans). BTF performance requires continuous monitoring of these bacteria at several sampling points. The Most Probable Number (MPN) technique is at the moment the method of choice to enumer...

Transient-state experiments with the obligately autotrophic Thiobacillus sp. strain W5 revealed that sulfide oxidation proceeds in two physiological phases, (i) the sulfate-producing phase and (ii) the sulfur- and sulfate-producing phase, after which sulfide toxicity occurs. Specific sulfur-producing characteristics were independent of the growth rate. Sulfur formation was shown to occur when t...