نتایج جستجو برای: throughput nucleotide sequencing
تعداد نتایج: 307622 فیلتر نتایج به سال:
Genetic species identification is often necessary for flocks, such as rockfishes in the genus Sebastes (Teleostei, Scorpaenidae). Traditional visual methods are challenged by presence of many sympatric rockfish with morphologically similar juveniles. Here we present a straightforward approach using 96 nuclear microhaplotype loci that can be efficiently genotyped high-throughput DNA sequencing. ...
An unprecedented use of high-throughput sequencing for routine monitoring SARS-CoV-2 viruses in patient samples has created a dataset over 6 million genomes. To monitor genomes, deposited the GISAID database, and to track continuous sequence evolution molecular assay oligonucleotide target sequences. A simple pipeline tool non-experts was developed mine this database nucleotide changes oligonuc...
Genetic structure can be altered by chemical mutagenesis, which is a common method applied in molecular biology and genetics. Second-generation sequencing provides a platform to reveal base alterations occurring in the whole genome due to mutagenesis. A model legume, Lotus japonicus ecotype Miyakojima, was chemically mutated with alkylating ethyl methanesulfonate (EMS) for the scanning of DNA l...
چکیده لاکتوز مهم ترین کربوهیدرات و اسمولیت (osmolit) اصلی در شیر می باشد. این ترکیب توسط هترودیمر پروتئینی لاکتوز سنتاز واقع در سلول های اپیتلیال غدد پستانی سنتز می گردد. لاکتوز سنتاز از دو جزء آلفا-لاکتالبومین (?-la) و بتا 1،4-گالاکتوزیل ترانسفراز (?4gal-t1) تشکیل شده است. در مطالعه ی حاضر کل ژن ?-la در نواحی کد شونده (اگزون) (exon) و غیر کد شونده (اینترون) (intron) 100 نمونه از گاوهای هولشتا...
DNA sequencing by synthesis (SBS) offers an approach for potential high-throughput sequencing applications. In this method, the ability of an incoming nucleotide to act as a reversible terminator for a DNA polymerase reaction is an important requirement to unambiguously determine the identity of the incorporated nucleotide before the next nucleotide is added. A free 3'-OH group on the terminal ...
New regulatory roles continue to emerge for both natural and engineered noncoding RNAs, many of which have specific secondary and tertiary structures essential to their function. Thus there is a growing need to develop technologies that enable rapid characterization of structural features within complex RNA populations. We have developed a high-throughput technique, SHAPE-Seq, that can simultan...
genetic identification of trichomonas vaginalis by using the actin gene and molecular based methods.
background: trichomonas vaginalis is the agent of urogenital tract infection that causes human trichomoniasis with some serious health complications. more understanding about genetic features of the parasite can be helpful in the study of the pathogenesis, drug susceptibility and epidemiology of the infection. for this end,we conducted analysis of the actin gene of t. vaginalis by applying the ...
The determination of the nucleotide sequence of a gene is often the ultimate goal of a research project and the frequency with which new sequences are described in the literature and computer databases is remarkable. The basic techniques that underpin these endeavours are remarkably simple and their description now frequently forms part of the very earliest portion of a biological science under...
High-resolution genome analysis technologies provide an unprecedented level of insight into structural diversity across crop genomes. Low-cost discovery of sequence variation has become accessible for all crops since the development of next-generation DNA sequencing technologies, using diverse methods ranging from genome-scale resequencing or skim sequencing, reduced-representation genotyping-b...
Virus discovery from high throughput sequencing data often follows a bottom-up approach where taxonomic annotation takes place prior to association to disease. Albeit effective in some cases, the approach fails to detect novel pathogens and remote variants not present in reference databases. We have developed a species independent pipeline that utilises sequence clustering for the identificatio...
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