The cellular mechanisms supporting plasticity during memory consolidation have been a subject of considerable interest. De novo protein and mRNA synthesis in several brain areas are critical, and more recently protein degradation, mediated by the ubiquitin-proteasome system (UPS), has been shown to be important. Previous work clearly establishes a relationship between protein synthesis and prot...

AIM To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells. METHODOLOGY Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evalu...

Several experimental manipulations result in axonal regeneration in the central nervous system (CNS) when applied before or at the time of injury but not when initiated after a delay, which would be clinically more relevant. As centrally injured neurons show signs of atrophy and degeneration, it raises the question whether chronically injured neurons are able to regenerate. To address this ques...

The ability to replicate physiological hemodynamic conditions during in vitro tissue development has been recognized as an important aspect in the development and in vitro assessment of engineered heart valve tissues. Moreover, we have demonstrated that studies aiming to understand mechanical conditioning require separation of the major heart valve deformation loading modes: flow, stretch, and ...

Background: Osteoporosis is a decrease in bone mass and worsening of tissue, which causes density or porous bones. Muscle strengthening exercise voluntary to increase skeletal muscles strength, power, endurance, that includes the use weight machines, hand-held weight, band, own body weight. People do for many purposes, including aesthetic, physical therapy, sports, conditioning sports performan...

Changes in tissue health were monitored in a group of spinal cord injury (SCI) individuals with the use of an implanted neuromuscular electrical stimulation (NMES) system to provide standing and to facilitate standing transfers. Tissue health was evaluated through monitoring tissue oxygen levels in the ischial region along with measuring interface pressures at the seating support interface. Bas...

Microfluidics technology has the potential to allow precise control of temporal and spatial aspects solute concentration, making it highly relevant for study volume transmission mechanisms in neural tissue. However, full utilization this depends on understanding how microfluidic flow at rates needed rapid solution exchange affects neuronal viability network activity. We designed a tape-based pr...

With their potent regenerative and protective capacities, stem cell-derived conditioned media emerged as an effective alternative to cell therapy, have a prospect be manufactured pharmaceutical products for tissue regeneration applications. Our study investigates the neuroregenerative potential of human dental pulp cells (DPCs) medium (CM) defines optimization strategy DPC-CM enhanced neuronal ...

This research was designed to study sexual differences in place conditioning induced by morphine in offspring born of female Wistar rats mated with drug-naïve males. Mothers were exposed to morphine during the 14th-16th days of gestational. Control dams were simply saline-injected. Female and male virgin offspring born of morphine-treated or saline-treated mothers were separately housed until b...