PURPOSE To examine the differential staining of two potential stress-response markers, alpha B-crystallin and the trabecular meshwork inducible glucocorticoid response protein (TIGR), in meshwork from normal and glaucomatous human eyes. METHODS Trabecular meshwork from 35 eyes from 23 donors with either primary open-angle glaucoma, pseudoexfoliative glaucoma, or low-tension glaucoma, and from...

Canaloplasty is a new nonperforating glaucoma operation, which utilizes the experience with viscocanalostomy and adds a further surgical step to keep the ostia open and to stretch the trabecular meshwork permanently. During the operation, a deep sclerectomy and viscocanalostomy are performed as described by Stegmann et al. After this, a 200-2m microcatheter (iTrack; iScience Surgical Corp, Menl...

UNLABELLED purpose To determine how primary human trabecular meshwork (HTM) cells are influenced by their interaction with nanopatterned substrates. METHODS HTM cells from several individuals were grown on planar or anisotropically ordered nanopatterned surfaces. Microscopy was used to measure cellular elongation and alignment. Cells were also incubated with 10(-7) M dexamethasone for compari...

We established and validated an in toto method to perform TdT-mediated dUTP nick end labeling to study apoptosis in human trabecular meshwork tissue obtained during trabeculectomy in glaucoma patients. In specimens from patients with primary open-angle glaucoma and primary angle-closure glaucoma, we detected a tendency for more apoptotic cells to accumulate in patients with primary open-angle g...