transgene integration and expression in host plant is quite unpredictable and is considered as the major problem in plant transformation. the variation in transgene copy number in transgenic plants influences the expression level and is one of such complication. in many plant species, the analysis of transgenic plants has shown that independent transgenic plants have one to many copies of trans...

abstract background: determination of transgenic embryos from non transgenic embryos sibling is an important step in producing homozygous transgenic mice. these steps need by pcr or southern blotting followed extraction of dna, but both techniques require skill and consume time. objective: the aim of this study was simulation of high accuracy method using novel enhanced green fluorescent protei...

The recombinant adeno-associated viral (rAAV) vector is one of the most effective vectors in gene therapy because its low immunogenicity, high transduction efficiency, broad tissue specificity, and long-term transgene expression ability. HEK293T cells are widely used to produce rAAV vectors, acquisition rAAV-producing essential processes for higher production. To acquire such cells, a method id...

The effect of an ovine growth hormone transgene on body growth and wool production to 12 mo of age of Merino sheep at pasture was investigated. There was no effect of the transgene on growth to 3 mo of age. From 7 mo onwards, animals heterozygous for the transgene began to grow faster and were significantly heavier than those without the transgene. The pattern of growth response was the same fo...

The exact site of transgene insertion into a plant host genome is one feature of the genetic transformation process that cannot, at present, be controlled and is often poorly understood. The site of transgene insertion may have implications for transgene stability and for potential unintended effects of the transgene on plant metabolism. To increase our understanding of transgene insertion site...

Transgenic mice carrying one complete copy of the human alpha 1(I) collagen gene on the X chromosome (HucII mice) were used to study the effect of X inactivation on transgene expression. By chromosomal in situ hybridization, the transgene was mapped to the D/E region close to the Xce locus, which is the controlling element. Quantitative RNA analyses indicated that transgene expression in homozy...

Precise control of transgene expression in a tissue-specific and temporally regulated manner is desirable for many basic and applied investigations gene therapy applications. This is important to regulate dose of transgene products and minimize unwanted effects. Previously described methods have employed tissue specific promoters, miRNA-based transgene silencing or tetR-KRAB-mediated suppressio...