A foundation for a DNA barcode reference library for the tropical plants of Australia is presented here. A total of 1572 DNA barcode sequences are compiled from 848 tropical Queensland species. The dataset represents 35% of the total flora of Queensland’s Wet Tropics Bioregion, 57% of its tree species and 28% of the shrub species. For approximately half of the sampled species, we investigated t...

DNA barcoding in the land plants presents a number of challenges compared to DNA barcoding in many animal clades. The CO1 animal DNA barcode is not effective for plants. Plant species hybridize frequently, and there are many cases of recent speciation via mechanisms, such as polyploidy and breeding system transitions. Additionally, there are many life-history trait combinations, which combine t...

BACKGROUND The plant working group of the Consortium for the Barcode of Life recommended the two-locus combination of rbcL+matK as the plant barcode, yet the combination was shown to successfully discriminate among 907 samples from 550 species at the species level with a probability of 72%. The group admits that the two-locus barcode is far from perfect due to the low identification rate, and t...

Aneura pinguis is a thalloid liverwort species with broad geographical distribution. It is composed of cryptic species, however, the number of cryptic species within A. pinguis is not known. Five cpDNA regions (matK, rbcL, rpoC1, trnH-psbA and trnL-trnF) and the entire nuclear ITS region were studied in 130 samples of A. pinguis from different geographical regions. The relationships between the...

Although DNA barcoding has become a useful tool for species identification and biodiversity surveys in plant sciences, there remains little consensus concerning appropriate sampling strategies and the treatment of indels. To address these two issues, we sampled 39 populations for nine Taxus species across their entire ranges, with two to three individuals per population randomly sampled. We seq...

Climate fluctuations in the Quaternary period significantly influenced evolution and distribution of plants, which caused to a serious genetic effects speciation activity [1]. Thus, Central Asia is distinguished by wide variety flora, including 9520 species, while 20% are endemics [2]. Great phenotypic diversity wild flora Uzbekistan, numbering about 4400 species vascular plants determined pecu...

Fritillaria Tourn. ex L. is a genus of Liliaceae including little more than 150 species occurring in the temperate Holarctic. sonnikovae Shaulo & Erst most recently described Siberian genus. In affinity section F. diagnosis, only dagana Turcz. and roylei Hook. are mentioned. Our study an original attempt to shed light on origin its evolutionary relationships with other using nuclear (ITS) p...

Abstract DNA barcoding allows the identification of an organism by comparing sequence selected regions (barcodes) with a previously compiled database, and it can be useful for taxonomic species in complex genera, such as Tamarix . Many this genus show convergent morphology, which leads to frequent errors their identification. Highly variable genetic markers, microsatellites or short repeats (SS...

A two-marker combination of plastid rbcL and matK has previously been recommended as the core plant barcode, to be supplemented with additional markers such as plastid trnH-psbA and nuclear ribosomal internal transcribed spacer (ITS). To assess the effectiveness and universality of these barcode markers in seed plants, we sampled 6,286 individuals representing 1,757 species in 141 genera of 75 ...