A part of the tRNA(Leu)(UAA) gene containing a 240-nucleotide group I intron was amplified by PCR from cyanobacterium Synechococcus PCC 6301 genomic DNA. The pre-tRNA synthesized from the cloned PCR product was efficiently self-spliced in vitro under physiological conditions. The gene encoding the tRNA(Leu)(UAA), trnL-UAA, was isolated from a Synechococcus PCC 6301 genomic library and the nucle...

In this study, based on molecular biology techniques, the matK and trnL-F regions of ten Annonaceae species grown in Binh Chau-Phuoc Buu Nature Reserve, Vietnam were successfully amplified sequenced for first time. Along with other highly homologous DNA sequences from GenBank database, phylogeny studied was also established. By using alignment tool NCBI percentage identity among presented. The ...

The current research deals with molecular systematics study of the genus Eryngium (Apiaceae) in Iraq. included characteristics traits chloroplast matK & trnL-F gene that have reliable value separate and determinate from others genera Apiaceae family. result reaches to investigated phylogenetic relation species belongs Erymgium using five in-group E. campestre L., creticum Lam., billardieri ...

The phylogeny of the species Epilobium in Kurdistan Region-Iraq was investigated by using six in-group species: E. anatolicum Hausskn. subsp. anatolicum, hirsutum L., obscurum Schreber, parviflorum rechingeri Raven., tetragonum L. and one out-group related genus: Ludwingia grandiflora (Michx.) Greuter & Burdet, based on trnL-F intergenic region chloroplast DNA internal transcribed spacer (I...

This study represents phylogenetic analyses of two woody polygonaceous genera Calligonum and Pteropyrum using both chloroplast fragment (trnL-F) and the nuclear ribosomal internal transcribed spacer (nrDNA ITS) sequence data. All inferred phylogenies using parsimony and Bayesian methods showed that Calligonum and Pteropyrum are both monophyletic and closely related taxa. They have no affinity w...

Calligonum is the only C4 genus within Polygonaceae. We applied DNA sequences from nuclear ribosomal internal transcribed spacer (nrITS) and five plastid genome regions (psbA-trnH, ycf6-psbM, trnL-F, rpl32-trnL rbcL) to reconstruct phylogeny of Calligonum. The nrITS combined were analysed separately. supports treatment mongolicum complex as a single species with intra-specific geographic struct...