Truncated green fluorescent protein (GFP) with the 11th β-strand removed is potentially interesting for bioconjugation, imaging, and the preparation of semisynthetic proteins with novel spectroscopic or functional properties. Surprisingly, the truncated GFP generated by removing the 11th strand, once refolded, does not reassemble with a synthetic peptide corresponding to strand 11 but does reas...

Adenosine Deaminases Acting on RNA (ADARs) have been studied in many animal phyla, where they have been shown to deaminate specific adenosines into inosines in duplex mRNA regions. In Drosophila, two isoform classes are encoded, designated full-length (contains the editase domain) and truncated (lacks this domain). Much is known about the full-length isoform, which plays a major role in regulat...

Scp160p is a 160 kDa RNA-binding protein in yeast previously demonstrated to associate with specific messages as an mRNP component of both soluble and membrane-bound polyribosomes. Although the vast majority of Scp160p sequence consists of 14 closely spaced KH domains, comparative sequence analyses also demonstrate the presence of a potential nuclear localization sequence located between KH dom...

In the present work, a study of thermoelastic analysis of a rotating thick truncated conical shell subjected to the temperature gradient and non-uniform internal pressure is carried out. The formulation is based on first-order shear deformation theory (FSDT), which accounts for the transverse shear. The governing equations, derived using minimum total potential energy principle, are solved, usi...

Several characterizations of a New Modified Weibull distribution, introduced by Doostmoradi et al. (2014), are presented. These characterizations are based on: (i) truncated moment of a function of the random variable (ii) the hazard function (iii) a single function of the random variable (iv) truncated moment of certain function of the 1st order statistic.

The full-length normal and T24 mutant human H-ras proteins and two truncated derivatives of the T24 mutant were expressed efficiently in Escherichia coli. The proteins accumulated to 1 to 5% of total cellular protein, and each was specifically recognized by anti-ras monoclonal antibodies. The two full-length proteins as well as a carboxyl-terminal truncated derivative (deleted for 23 amino acid...

Null mice for the dopamine D2 receptor (D2R) have been instrumental in understanding function of this protein. For our research, we obtained functional D2R knockout mouse strain described initially 1997. Surprisingly, biochemical characterization showed that is not a true knockout. We determined by sequence analysis rapid 3′ amplification cDNA ends express transcripts lack only eighth exon. Fur...

Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile entero...

The present study was aimed to construct a fusion plasmid harboring the extracellular domain of the influenza A M2-protein (M2e), which was fused to the N-terminus of the truncated HSP70 (HSP70359–610) molecule as a new approach for future vaccine research against influenza A. The amplified fragments, M2e and HSP70359-610 genes, were gel-purified. The products were then single digested with Bam...