Background: This study aimed to explore the efficacy of trypsin inhibitors in treatment severe pancreatitis (SP) by meta-analysis.

The inhibition of digestive enzyme trypsin by polyphenolic compounds derived from mountainous herbal infusions, as well the effect polarity and acidity media on this process were studied virtue UV-Vis absorption spectroscopy at 293.15 K. Mountainous infusions (thyme, peppermint) are known due to their biomedical significance wide use a beverage. binding constant (Kb) standard Gibbs energy chang...

The mechanism of the insulin-like action of trypsin was studied. When fat cells were exposed to trypsin (1 mg per ml) for 15 set, glucose metabolism in the cells was stimulated. This stimulation (like that by insulin) was inhibited by phloretin or 3-O-methyl-n-glucose. By the above treatment with trypsin, cellular lipolysis induced by epinephrine was blocked to a large extent, but that induced ...

Tetracycline (TC) and chlortetracycline (CTC) are common members of the widely used veterinary drug tetracyclines, the residue of which in the environment can enter human body, being potentially harmful. In this study, we establish a new strategy to probe the binding modes of TC and CTC with trypsin based on spectroscopic and computational modeling methods. Both TC and CTC can interact with try...

Digestive trypsins undergo proteolytic breakdown during their transit in the human alimentary tract, which has been assumed to occur through trypsin-mediated cleavages, termed autolysis. Autolysis was also postulated to play a protective role against pancreatitis by eliminating prematurely activated intrapancreatic trypsin. However, autolysis of human cationic trypsin is very slow in vitro, whi...

The mechanism of the insulin-like action of trypsin was studied. When fat cells were exposed to trypsin (1 mg per ml) for 15 set, glucose metabolism in the cells was stimulated. This stimulation (like that by insulin) was inhibited by phloretin or 3-O-methyl-n-glucose. By the above treatment with trypsin, cellular lipolysis induced by epinephrine was blocked to a large extent, but that induced ...

From purulent cystic fibrosis (CF) sputum, previous investigators partially purified a trypsinlike protease. A similar purified enzyme is available commercially as "human sputum trypsin." To explore the nature and origin of this preparation, we purified and NH2 terminally sequenced its major protein component. The resulting sequence, Ile-Val-Gly-Gly-Tyr-Thr-(Cys)-Ala-Ala-Asn-Ser-Val/Ile-Pro-Tyr...

Present study was conducted to characterize soybean genotypes for the kunitz trypsin inhibitor at molecular and biochemical level. Genotypes used in the present study included one null KTI soybean line PI542044 (the source of null allele of kunitz trypsin inhibitor being used in soybean breeding programs in India), DSRtiti (homozygous recessive genotype for KTi gene) and seven other elite soybe...

Early trypsin is a female-specific protease present in the midgut of the yellow fever mosquito Aedes aegypti during the first 4-6 h after ingestion of a blood meal. Transcription of the early trypsin gene occurs after adult emergence under control of juvenile hormone, but the transcript remains untranslated before feeding. Early trypsin was in vitro translated using mRNA extracted from midguts ...

Trypsin in duodenal aspirate and pure pancreatic juice samples has been measured by both radioimmunoassay and enzymatic mathods. The radioimmunossay has been shown to be specific and to detect trypsin in the presence of aprotinin (Trasylol). In duodenal juice samples from control subjects and from patients with primary biliary cirrhosis a good correlation was obtained between both immunoreactiv...