in this study, 32 multiparous non lactating holstein cows were used to investigate the effects of chromium-l-methionine (cr-met) supplementation and dietary energy source on production and nutrient digestibility during the periparturient period. cows were fed a total mixed ration from 28 days before anticipated calving through 28 days after calving. the study was designed as a randomized comple...

Eighteen multiparous early lactation Holstein cows were assigned to a replicated 3 × 3 Latin square with 28-d periods to evaluate milk protein production potential when corn silage (CS) or small grain silage (SGS) was added to diets based on alfalfa silage (AS). Treatments consisted of all AS, a mixture of AS and CS, or a mixture of AS and SGS. Treatment diets were isonitrogenous, isofibrous, a...

The hyperinsulinemic-euglycemic clamp (EGC) technique was used to investigate the effects of calcium salts of long-chain fatty acids (LCFA-Ca) and rumen-protected Met (RPM) on insulin sensitivity in the peripheral tissues of lactating cows. Six multiparous Holstein cows were used in a 3 × 3 Latin square experiment in each 14-d period. Dietary treatments were 0 (RPM0), 20 (RPM20), and 60 (RPM60)...

The effect of parity (multiparous vs primiparous) and body condition score (BCS; <3.0 or > or =3.0, lean vs fat) at parturition on metabolic and endocrine profiles from 1 month before to 2 months after parturition were studied in 42 Holstein cows grazing on improved pastures. BCS and milk production were determined every 2 weeks. Non-esterified fatty acids (NEFA), beta-hydroxy-butyrate (BHB), i...

This study aimed to determine if 200 μg of GnRH (gonadorelin hydrochloride) would increase ovulatory response and pregnancies per AI (P/AI) compared with 100 at the first breeding-Ovsynch a Double-Ovsynch program (DO) in lactating Holstein cows. Weekly cohorts primiparous (n = 719) multiparous 1,191) cows submitted DO [GnRH – 7 d PGF2α 3 (G1) (PG1) 1 ~32 h (G2) ~16 TAI] for timed-artificial ins...

Trials were conducted to evaluate effects of a fermentation extract of Aspergillus oryzae (AO) on milk production and composition, diet digestibility, and rectal temperature changes in lactating dairy cows. Treatments were incorporated as a top dressing at the morning feeding and consisted of control (90 g/d of ground sorghum) or AO (3 g of culture + 87 g of ground sorghum daily). Twenty-four m...

The objective of this study was to evaluate the effects of replacing protein sources that are commonly used in North America with triticale dried distillers grains with solubles (TDDGS) as a source of dietary N on the productivity of lactating dairy cows. Experimental diets contained 17.1% TDDGS, 17.5% Corn DDGS (CDDGS), 13.1% canola meal (CM), or 10.2% soybean meal (SBM) on a DM basis so that ...

The objective of this study was to investigate the effect of different body condition score (BCS) at 30 days before calving (-30 days) induced by a differential nutritional management from -100 days until -30 days on productive parameters, the interval to first ovulation and blood parameters in primiparous and multiparous Holstein cows under grazing conditions until 60 days post partum. The exp...

The objective of this study was to compare utilization of red clover (Trifolium pratense L.) and alfalfa (Medicago sativa L.) by lactating dairy cows. Red clover and alfalfa were harvested and conserved as silage at two maturities in 2 consecutive yr. Each year, diets containing experimental forages and supplemental grain were fed to 16 multiparous Holstein cows in early lactation in a replicat...

Increased Milk Production, Concentration of Unsaturated Fatty Acids and Conjugated Linoleic Acid in the Holstein Lactating Cows using Monensin