Many G-protein-coupled receptors, including the alpha(1b)-adrenoceptor, form homo-dimers or oligomers. Mutation of hydrophobic residues in transmembrane domains I and IV alters the organization of the alpha(1b)-adrenoceptor oligomer, with transmembrane domain IV playing a critical role. These mutations also result in endoplasmic reticulum trapping of the receptor. Following stable expression of...

Objective(s): The protective effect of regular running on sleep deprivation (SD)-induced cognitive impairment has been revealed. In this study, we focused on the effects of regular exercise, sleep deprivation and both of them together on the microRNA-1b (miR-1b) expression and their relation to the behavioral parameters and brain-derived neurotrophic factor (BDNF) expr...

Using a yeast two-hybrid method, we searched for amyloid precursor protein (APP)-interacting molecules by screening mouse and human brain libraries. In addition to known interacting proteins containing a phosphotyrosine-interaction-domain (PID)-Fe65, Fe65L, Fe65L2, X11, and mDab1, we identified, as a novel APP-interacting molecule, a PID-containing isoform of mouse JNK-interacting protein-1 (JI...

Accumulating evidence has demonstrated that microRNAs (miRNAs) play critical roles in cancer initiation and development by functioning either as oncogenes or as tumor suppressors. The role of microRNA-449a (miR-449a) in endometrial cancer remains unclear. We examined the levels of miR-449a and miR-449b in benign endometrium, type I and type II endometrial cancer tissues by quantitative real-tim...

RasGRPs, which load GTP onto Ras and Rap1, are expressed in vertebrate and invertebrate neurons. The functions, regulation, and mechanisms of action of neuronal RasGRPs are unknown. Here, we show how C. elegans RGEF-1b, a prototypical neuronal RasGRP, regulates a critical behavior. Chemotaxis to volatile odorants was disrupted in RGEF-1b-deficient (rgef-1⁻/⁻) animals and wild-type animals expre...

Aim It was previously reported that several patients with pseudohypoparathyroidism type 1b (PHP-1b) have a more generalised imprinting defect. However there was no report that a patient of PHP-1a has any generalised imprinting defects. Here we aim to report a case of PHP-1a with hypomethylation at the Kv-differentially methylated region (DMR) (11p15.5), responsible for Beckwith-Wiedemann syndrome.

background: the hepatic secretion of apolipoprotein b (apob), containing lipoproteins, is known to be regulated by insulin, and the overproduction of these atherogenic lipoproteins occurs in insulin-resistant states. protein tyrosine phosphatase 1b (ptp-1b) is a key regulator of hepatic insulin signaling and is also upregulated in insulin resistance. we aimed to investigate the role of ptp-1b i...

The purpose of the present study was to investigate the role of P-glycoprotein (P-gp) in drug disposition in skin. The distribution of P-gp substrates (rhodamine 123 and itraconazole) to the skin after administration from the epidermal side was lower in P-gp gene knockout (mdr1a/1b(-/-)) mice than that in wild-type mice. Coadministration of propranolol, a P-gp inhibitor, decreased the distribut...

In this study, we have categorized cord blood CD5(+) B cells, which were examined using the horseradish peroxidase-colloidal gold double labeling immunoelectron microscopy, into three subtypes based on their morphology and immunohistochemical characteristics. Type 1a cells and type 1b cells (9% and 17% of the CD5(+) B cells, respectively) had few cytoplasmic organelles, a high nuclear/cytoplasm...