The determination of unknown DNA sequences around a known locus has important applications in molecular genetics, specifically in genomic walking and genome mapping. Several PCR-based methods have been reported to address this issue, but they often involve multiple, time-consuming steps. We have previously described a technique known as restriction site PCR (RS-PCR) that allows sequence acquisi...

A recent outbreak of hand, foot, and mouth disease in Singapore in 2000 affected several thousand children and resulted in four deaths. The aim of this study was to determine the applicability of reverse transcription-PCR (RT-PCR) with universal pan-enterovirus primers and enterovirus 71 (EV71) type-specific primers for the direct detection of enteroviruses in clinical specimens derived from th...

A collaborative study initiated from the first annual European Union (EU) Concerted Action Group meeting (project PL 98-4032, 5-7 May 1999, Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany) was organised between the Veterinary and Agrochemical Research Centre in Brussels, the Federal Research Centre in Tübingen and the OIE/FAO World Reference Laboratory for Foot-and-Mout...

Insect mitochondrial genome (mtDNA) analysis is a powerful tool for the study of population genetics and phylogenetics. In the past few years primer sequences for the PCR amplification of various insect mtDNA genes have been published. The objectives of this study were (1) present new primer sequences for six insect mitochondrial genes and (2) test primers designed in our laboratory and some pr...

coagulase-positive and coagulase negative, methicillin-resistant staphylococci are major causes of serious nosocomial infections and it is very important to have a reliable test to detect these bacteria. a multiplex polymerase chain reaction (mpcr) was used on 100 clinical samples for simultaneous amplification of the universal bacterial, mec-a encoding the penicillin binding protein 2a, which ...

Quantitative real-time PCR has become the method of choice for measuring mRNA transcription. Recently, fast PCR protocols have been developed as a means to increase assay throughput. Yet it is unclear whether more rapid cycling conditions preserve the original assay performance characteristics. We compared 16 primer sets directed against Epstein-Barr virus (EBV) mRNAs using universal and fast P...

The present study aimed to evaluate the PCR assays (using Universal and specific primers) for detection of the major pathogens of bubaline subclinical mastitis directly from 160 buffaloes milk samples compared to bacterial examination. The bacteriological examinations showed incidence of 23.75%, 15.62%, 3.12% and 0.62% for E. coli, S. aureus, S. agalactiae and S. dysgalactiae respectively. PCR ...

http://bfw.ac.at/200/1859.html The data collected: A data base is presented which collects published primer information for chloroplast DNA. Additional primers were designed in order to fill gaps were no or little primer information could be found. Chloroplast genes evolve slowly, and many primers have been designed to work across species ('universal primers', e.g. Taberlet et al. 1991, Grivet ...

armillaria root and crown rot is an important disease of woody plants including fruit, forest and ornamental trees in different regions. the disease is common in isfahan and causes economical losses on a broad range of fruit trees. there only a few tolerant varieties to disease, and there is no efficient method in controlling the disease. therefore, detection of the pathogen from soil is necess...

a water snake (natrix natrix) was referred to the small animal teaching hospital, faculty of veterinary medicine, university of tehran. it had two subcutaneous masses. radiographic and ultrasonographic images revealed the presence of two subcutaneous abscesses. the abscesses were removed surgically and specimens were examined by conventional microbial culture and multiplex pcr. inv-a universal ...