introduction and aims: omeprazole is a gastric parietal cells proton pump inhibitor that is also active against h. pylori in vitro. this study was designed to examine the neutralization of h. pylori cytotoxicity on vero cells by omeprazole micronized in strains isolated from gastritis, ulcer, cancer and barrett's ulcer, to determine whether omeprazole can inhibit vacuolation of the vero cells i...

Peptic ulceration is among the most prevalent gastrointestinal disorders characterized by pepsin and gastric acid mediated mucosal damage, as result of imbalance between defensive and offensive processes. The main objective of the current study was to investigate the antiulcer potentials of Polygonum hydropiper crude methanolic ectract (Ph.Cr) in aspirin induced ulcerogenesis using pylorus liga...

Different parts of Acacia nilotica (L.) Delile, Calotropis procera (Aiton) W.T. Aiton, Adhatoda vasica Nees, Fagoniaar abica L. and Casuarina equisetifolia L. are traditionally used in folk medicine for the treatment of a variety of common ailments like nausea, cold, cough, asthma, fevers, diarrhea, sore throat, swelling, etc. The present study was aimed to evaluate the anti-Helicobacter pylori...

We assayed the in vivo activity of the ureases of soybean (Glycine max) embryos by genetically eliminating the abundant embryo-specific urease, the ubiquitous urease, or a background urease. Mutant embryos accumulated urea (250-fold over progenitor) only when lacking all three ureases and only when developed on plants lacking the ubiquitous urease. Thus, embryo urea is generated in maternal tis...

Urease inhibitor [N-(n-butyl) thiophosphoric triamide (NBPT)] and nitrification (NI) (3,4-dimethylpyrazole phosphate) have been used to reduce nitrogen (N) losses from urea-based fertilizers. This study evaluated the effect of temperature, NBPT, NI on kinetic thermodynamic properties urea hydrolysis in six soils. Soils were amended (250 kg N·ha ?1 ) with (UR), NBPT treated (UR ), or + DI incuba...

Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, d...

The acetohydroxamic acid (AHA), a potent inhibitor of urease, inhibits soybean urease competitively and reversibly. The I 50 and K i value for AHA were 900 M and 0.053 mM, respectively at pH 7.0, 37 °C. The variation in pH over the pH 6 9 affected K i and therefore binding of AHA in the active site. The affinity of AHA for the active site decreases with lowering of pH (below the pK a value of ...