Objective: This study examined the association of homocysteine and uric acid with estimated glomerular filtration rate (eGFR) and explored their pathophysiological significance in patients with type 2 diabetes mellitus. Methods: We performed a cross-sectional study in 56 Japanese patients with type 2 diabetes. The correlations of serum total homocysteine level with age, diabetes duration, HbA1c...

AIM To investigate whether intra-personal variation in serum uric acid concentration is influenced by genes that were described to be associated with serum uric acid levels in cross-sectional studies. METHODS The study included 92 participants from the isolated community of the Croatian island of Vis. For each participant, two uric acid concentration measurements were available, one from 2002...

OBJECTIVE To evaluate the association between serum uric acid and obesity among university students who participated in routine health screening in 2013. METHODS In this cross-sectional study, 3529 subjects were analyzed. Obesity categories were classified by BMI levels references in China. And serum uric acid levels were classified by serum uric acid quartiles. Two-sample T-test and Wilcoxon...

Recent data suggest that uric acid is generated locally in the vessel wall by the action of xanthine oxidase. This enzyme, activated during ischemia/reperfusion by proteolytic conversion of xanthine dehydrogenase, catalyzes the oxidation of xanthine, thereby generating free radicals and uric acid. Because of the potential role of ischemia/reperfusion in vascular disease, we studied the effects ...

Proteins, fats, and carbohydrates or their metabolic derivatives have been investigated in great detail as possible precursors of the carbon chain of uric acid (3-11). The usual procedure has been to test the effect of the administration of these substances on the excretion of uric acid under a variety of experimental conditions. For the most part, experiments have been performed on the fasted ...

We describe our optimization and evaluation of a candidate Reference Method for uric acid in serum. Reaction parameters were optimized for a manual, enzymic method for uric acid in which highly purified microbial uricase is used to quantitate uric acid by a differential ultraviolet procedure. We evaluated the method in terms of freedom from interferences, analytical recovery, precision, and com...

Although most investigators ( 1 to 4) have agreed that the ingestion of protein increases the output of uric acid in man, there has been no clear elucidation of the mechanism whereby this is accomplished. Mares (5) believed that the increased uric acid in urine following high protein feeding resulted from the deterioration of nuclei of digestive cells concerned in the assimilation of the ingest...

Uric acid is a product of metabolic breakdown of purine nucleotides. In humans, uric acid is the final oxidation product of purine catabolism (1). Elevated serum uric acid (hyperuricemia) is closely associated with visceral far accumulation (2-4) and various metabolic disorders such as glucose intolerance, elevated blood pressure, dyslipidemia and atherosclerotic cardiovascular diseases, concep...

Uric acid is involved in nitrogenous waste in animals, together with ammonia and urea. Uric acid has also antioxidant properties and is a surrogate marker of metabolic syndrome. We observed that the elevated plasma uric acid of high-fat fed mice was normalized by benzylamine treatment. Indeed, benzylamine is the reference substrate of semicarbazide-sensitive amine oxidase (SSAO), an enzyme high...

Accumulating clinical evidence suggests that hyperuricemia is associated with an increased risk of type 2 diabetes. However, it is still unclear whether elevated levels of uric acid can cause direct injury of pancreatic β-cells. In this study, we examined the effects of uric acid on β-cell viability and function. Uric acid solution or normal saline was administered intraperitoneally to mice dai...