BACKGROUND Pathogenic strains in Escherichia coli can be divided into several pathotypes according to their virulence features. Among them, uropathogenic E. coli causes most of the urinary tract infections and has a genotype distinct from other virulent strains of E. coli. In this study, we sequenced and analyzed the genome of E. coli NCCP15653 isolated from the feces of a diarrhea patient in 2...

There are many different bacterial infections in the world that patients are suffering from and research teams are trying to find suitable ways to prevent and treat them. Urinary Tract Infections (UTIs) are most important infections in the world , and they are more common among women because vaginal cavity is near to urethral opening. The aim of this study is cloning of synthetic gene include a...

antibiotic resistance is a phenomenon in which antibiotic used to treat bacteria becomes useless due to resistance mechanism. increased drug resistance and occurrence of multiple drug resistance in bacteria specificity nosocomial and urinary tract infection bacteria has reduced the possibilities of treating these infectious diseases. efflux pumps are one of the major mechanism of mdr in bacteri...

background escherichia coli is one of the most causative pathogen of urinary tract infection. urinary tract infections (utis) are the second most common cause of morbidity and remain a serious health concern among the clinicians. the severity of uti caused by uropathogenic e. coli (upec) is due to the expression of a wide spectrum of virulent factors such as adhesin coding operons. little is kn...

conclusions these results indicate that the pcr method is a powerful genotypic assay for the detection of adhesin-encoding operons. thus, this assay can be recommended for clinical use to detect virulent urinary e. coli strains, as well as epidemiological studies. materials and methods a total of 100 upec isolates were obtained from urine samples of patients with uti. the prevalence of 5 virule...

TcpC, a new Toll/interleukin-1 receptor domain-containing protein of uropathogenic Escherichia coli involved in the suppression of innate immunity, was found in 2008. The aim of the present study was to determine the prevalence of tcpC and its association with virulence factors and phylogenetic groups among strains from a collection of 212 E. coli isolates from urinary tract and skin and soft t...

In this report, we analyzed the prevalence of the sat gene in 336 Escherichia coli samples collected from stools of children with and without diarrhea in Brazil and in 100 uropathogenic E. coli strains. The results show a high correlation between diffusely adhering E. coli (DAEC) and the presence of sat (44%) in intestinal isolates. DAEC strain FBC114 expresses a 107-kDa protein, which showed 9...

BACKGROUND AND OBJECTIVES Bacteria need iron for growth and most of them can actively acquire Fe ions using especial iron-chelating proteins which named siderophores. We aimed to determine the frequencies of iucA, iroN and irp2 genes in the uropathogenic Escherichia coli (UPEC) isolates. We also analyzed the effects of siderophore genes beside iron supplements on growth rate of the isolates. ...

Studies of Uropathogenic Escherichia coli (UPEC) pathogenesis have relied heavily on genetic manipulation to understand virulence factors. We applied a recently reported positive-negative selection system to create a series of unmarked, scarless FimH mutants that show identical phenotypes to previously reported marked FimH mutants; these are now improved versions useful for definitive assignmen...