BACKGROUND/AIMS Invasive infection with emerging moulds is increasing in incidence and reliable methods for speciating these organisms in tissue sections need to be developed. METHODS Two methods for extracting fungal DNA from paraffin wax embedded tissue sections, based on the TaKaRa DEXPAT kit and QIAamp DNA mini kit, were optimised and compared. DNA was amplified by PCR using pan-fungal pr...

BACKGROUND Current diagnosis of Helicobacter pylori infection by biopsy-based tests requires invasive sampling. Non-invasive methods such as the H. pylori stool-antigen (HpSA) test may be the best alternative for diagnosis of active infection. However, due to the presence of antigenic-diversity among the strains, various commercial tests have shown some discrepancies in different geographical-a...

There has recently been a proliferation of commercial kits available for apoproteins A-I and B. Since reference procedures for apoproteins have not yet been established we have elected to compare apoprotein kit methods with highly standardized apoprotein B and A-I radioimmunoassays developed at the Northwest Lipid Research Center. Commercial radial immunodiffusion kits for apoproteins A-I and B...

Experiments were performed to evaluate five commercial kit assays used for the detection of antibodies to dsDNA. The kits were compared using a performance index score as recommended by the guidelines of the European Committee for Clinical Laboratory Standards. The highest performance score was obtained using the radioimmunoassay from Immunodiagnostic Services Ltd, with the Amersham kit second,...

AIM To show that application of the polymerase chain reaction (PCR) method modified for amplification of a low-copy number DNA samples, ie, the isolation of PCR products (IPCRp), would represent improvement in obtaining genotypes from a fecal DNA compared with previously used genotyping methods. METHODS The DNA from the horse fecal matter was extracted by modified Qiagen DNA Stool Mini Kit pr...

A new rapid diagnostic test for detection of influenza A virus was evaluated with four sets of experiments: first, a comparison with a commercial diagnostic kit against a panel of virus strains was conducted; second, the kit was tested against a collection of 40 strains of influenza A virus isolated from five different host species and 26 strains of other respiratory viruses used as controls; t...

Six commercial preparations of human genomic DNA were quantified using six quantification methods including UV spectrometry, SYBR-green dye staining, slotblot hybridization with the probe D17Z1, and three TaqMan real-time PCR assays: Quantifilerk Human DNA Quantification kit, Quantifilerk Y DNA Quantification kit, and RB1 rt-PCR. In general, all methods measured higher DNA concentrations than e...

The applicability of a commercial human rotavirus detection kit for the detection of lapine rotavirus in laboratory rabbits was examined. Rotavirus antigen positive samples determined by the kit were shown to include lapine rotavirus by reverse transcriptional polymerase chain reaction and restriction endonuclease analysis. The kit was confirmed to be adequate for the detection of lapine rotavi...

introduction: radiolabeled antibiotics are being used for the specific diagnosis of infection by exploiting their specific binding properties to the bacterial components, thereby making it possible to differentiate infection from sterile lesions. the aim of this work was to prepare and evaluate a freeze-dried kit of ciprofloxacin designed for the labeling with 99mtc. methods: factors like amou...

OBJECTIVE To evaluate the accuracy of a commercial blood transfusion cross-match kit when compared to the standard laboratory method for establishing blood transfusion compatibility. DESIGN A prospective observational in intro study performed from July 2009 to July 2013. SETTING Private referral veterinary center. ANIMALS Ten healthy dogs, 11 anemic dogs, and 24 previously transfused dogs...