نتایج جستجو برای: using rnease mini kit

تعداد نتایج: 3426840  

2018
Lisa F. Stinson Jeffrey A. Keelan Matthew S. Payne

The establishment of human gut microbiota commences initially in utero. Meconium-the first fecal material passed after birth-can be used to study fetal gut contents; however, processing meconium samples for microbiome studies presents significant technical challenges. Meconium hosts a low biomass microbiome, is tar-like in texture and contains high concentrations of PCR inhibitors. This study a...

Hossein Safari, Nasibe Alipour

Solar mini-dimmings can be detect in the Extreme Ultra-Violet coronal eruptions. Here, sequences of 171_A images taken by Solar Dynamic Observatory/Atmospheric Imaging Assembaly on 13 June 2010 are used. In this special day, both of coronal mass ejection and prominence were observed. The average velocities and accelerations of 500 mini-dimmings which were detected using on feature based classif...

پایان نامه :وزارت بهداشت، درمان و آموزش پزشکی - دانشگاه علوم پزشکی و خدمات بهداشتی درمانی مشهد - دانشکده دندانپزشکی 1390

عنوان بررسی بروز پروتئین c-kit (cd117) در چهار تومور خوش خیم و بدخیم شایع غدد بزاقی مقدمه و هدف پلئومورفیک آدنوما، آدنوئید سیستیک کارسینوما، پلی مورفوس low-grade آدنوکارسینوما، و موکواپیدرموئید کارسینوما ازجمله نئوپلاسم های شایع غدد بزاقی می باشند که با پیش آگهی های بسیار متفاوتی همراه می باشند. گاهی افتراق این نئوپلاسم ها از یکدیگر به دلیل همپوشانی های هیستوپاتولوژیک می تواند مشکل باشد.c-ki...

Journal: :iranian journal of parasitology 0
j abdi department of medical parasitology & mycology, school of public health, tehran university of medical sciences, tehran, iran b kazemi department of parasitology, shahid beheshti university, m.c., tehran, iran and cellular and molecular biology research center, shahid beheshti university, m.c., tehran, iran a haniloo department of medical parasitology & mycology, school of medicine, zanjan university of medical sciences, zanjan, iran m mohebali department of medical parasitology & mycology, school of public health, tehran university of medical sciences, tehran, iran m mahmoudi department of epidemiology and biostatistics, medical parasitology & mycology, school of public health, tehran university of medical sciences, tehran, iran s rezaei department of medical parasitology & mycology, school of public health, tehran university of medical sciences, tehran, iran

background: regarding that accurate diagnosis of human hydatidosis still needs more investigations, the present study was conducted to clone, express, and evaluate the gene encoding agb subunits (egagb16 kda) from echinococcus granulosus (iranian g1 strain) and its evaluation by elisa test. methods: dna was extracted from protoscoleces and was utilized by pcr for strain identification. total rn...

Journal: :Genetics and molecular research : GMR 2011
J Kasaian J Behravan M Hassany S A Emami F Shahriari M H Khayyat

The genus Juniperus L. (Cupressaceae), an aromatic evergreen plant, consists of up to 68 species around the world. We classified five species of Juniperus found in Iran using molecular markers to provide a means for molecular identification of Iranian species. Plants were collected (three samples of each species) from two different provinces of Iran (Golestan and East Azarbayejan). The DNA was ...

Journal: :iranian red crescent medical journal 0
mahsa tabibnejad department of immunology and microbiology, arak university of medical sciences, arak, ir iran mohammad yousef alikhani brucellosis research center, hamadan university of medical sciences, hamadan, ir iran; brucellosis research center, hamadan university of medical sciences, hamadan, ir iran. tel: +98-9125443147, fax: +98-8132533936, e-mail:; mohammad arjomandzadegan, tuberculosis and pediatric infectious research center and department of microbiology, arak university of medical sciences, arak, ir iran. tel: +98-9188602576, fax: +98-8634173518 mohammad arjomandzadegan tuberculosis and pediatric infectious research center and department of microbiology, arak university of medical sciences, arak, ir iran; brucellosis research center, hamadan university of medical sciences, hamadan, ir iran. tel: +98-9125443147, fax: +98-8132533936, e-mail:; mohammad arjomandzadegan, tuberculosis and pediatric infectious research center and department of microbiology, arak university of medical sciences, arak, ir iran. tel: +98-9188602576, fax: +98-8634173518 seyed hamid hashemi brucellosis research center, hamadan university of medical sciences, hamadan, ir iran zahra naseri blood transfusion research center, high institute for research and education in transfusion, hamadan, ir iran

conclusions the results prove that the presented double pcr method could be used to detect positive cases from culture-negative blood samples. the chelex 100 method is simpler and safer than the use of qiagen kit for dna extraction. materials and methods a total of 100 patients with suspected brucellosis were included in this experimental study and given positive serological tests. diagnosis wa...

Journal: :Journal of clinical microbiology 2003
Daniel R Kuritzkes Robert M Grant Paul Feorino Marshal Griswold Marie Hoover Russell Young Stephen Day Robert M Lloyd Caroline Reid Gillian F Morgan Dean L Winslow

The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mutations and to determine the effects of RNA extraction methods, anticoagulants,...

Journal: :Forensic science international. Genetics 2012
Dyan J Daly Charlotte Murphy Sean D McDermott

The transfer of DNA from hands to objects by holding or touching has been examined in the past. The main purpose of this study was to examine the variation in the amount of DNA transferred from hands to glass, fabric and wood. The study involved 300 volunteers (100 for glass, 100 for fabric and 100 for wood) 50% of which were male and 50% female. The volunteers held the material for 60s. The DN...

Journal: :سالمند 0
مژده میرعارفین mojde mirarefin tehran university of medical sciences and healthservices, tehran, iran.دانشگاه علوم پزشکی و خدمات بهداشتی درمانی تهران، فرشاد شریفی farshad sharifi tehran university of medical sciences and healthservices, tehran, iran.دانشگاه علوم پزشکی و خدمات بهداشتی درمانی تهران، ندا نظری neda nazari university of social welfare and rehabilitationsciences, tehran, iran.دانشگاه علوم بهزیستی و توانبخشی حسین فخرزاده hossein fakhrzedeh kahrizak charityfoundation, tehran, iran.آسایشگاه خیریه کهریزک مریم قادرپناهی maryam ghaderpanahi tehran university of medical sciences and healthservices, tehran, iran.دانشگاه علوم پزشکی و خدمات بهداشتی درمانی تهران، زهره بادامچی زاده zohre badamchizade tehran university of medical sciences and healthservices, tehran, iran.دانشگاه علوم پزشکی و خدمات بهداشتی درمانی تهران،

objectives: the aims of this study were to investigate the association of mini-nutritional assessment and activities of daily living as well as examining mini nutritional assessment as a good prognostic tool ofactivities of daily living in elderly residents of nursing home. methods & materials: in a cross-sectional study, among 1200 recorded files, 247 subjects completed mini nutritional assess...

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