background: hepatitis c is a global health problem. the exact mechanisms by which hepatitis c virus (hcv) can evade the host immune system have become controversial. whether hcv polyproteins modulate ifn signalling pathways or hcv proteins are responsible for such a property is the subject of interest. therefore, an efficient baculovirus delivery system was developed to introduce the whole geno...

The genome of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) has an untranslated region (UTR) that enhances the replication and transcription RNA, subgenomic RNA (sgRNA) which encodes 4 structural proteins are essential for viral replication. host cell SARS CoV-2 expresses important enzyme called TMPRSS2 protease allows virus to invade cell. Peptide conjugated phosphorodiamidate m...

All polyadenylated mRNAs contain sequence of variable length between the coding region and the poly(A) tail. Little has been done to establish what role the length of the 3' untranslated region (3'UTR) plays in posttranscriptional regulation. Using firefly luciferase (luc) reporter mRNA in transiently transfected Chinese hamster ovary (CHO) cells, we observed that the addition of a poly(A) tail...

The 3′-untranslated region (3′-UTR) of PD-L1 is significantly longer than the coding sequences (CDSs). However, its role and regulators have been little studied. We deleted whole 3′-UTR by CRISPR-Cas9. Prognostic analysis was performed using online tools. Immune infiltration Timer Xcell packages. Immunotherapy response prediction Cox regression R software. MicroRNA network conducted Cytoscape l...

The synthesis of plus and minus RNA strands of several RNA viruses requires as a first step the interaction of some viral regulatory sequences with cellular and viral proteins. The dengue 4 virus genome, a single-stranded, positive-polarity RNA, is flanked by two untranslated regions (UTR) located in the 5' and 3' ends. The 3'UTR in the minus-strand RNA [3'UTR (-)] has been thought to function ...

HLA-DRB1 is the most polymorphic MHC (major histocompatibility complex) class II gene in human, and plays a crucial role in the development and function of the immune system. Extensive polymorphisms exist in the promoter and 3'-UTR of HLA-DRB1, especially a LTR (Long terminal repeat) element in the promoter, which may be involved in the expression regulation. However, it remains unknown how the...

Apurinic/apyrimidinic endonuclease 1 (APEX1) is a multifunctional protein which plays a central role in the BER pathway. APEX1 gene being highly polymorphic in cancer patients and has been indicated to have a contributive role in Apurinic/apyrimidinic (AP) site accumulation in DNA and consequently an increased risk of cancer development. In this case-control study, all exons of the APEX1 gene a...

All polyadenylated mRNAs contain sequence of variable length between the coding region and the poly(A) tail. Little has been done to establish what role the length of the 3* untranslated region (3*UTR) plays in posttranscriptional regulation. Using firefly luciferase (luc) reporter mRNA in transiently transfected Chinese hamster ovary (CHO) cells, we observed that the addition of a poly(A) tail...