TiO2 nanostructured films were synthesized by an aerosol chemical vapor deposition (ACVD) method with different controlled morphologies: columnar, granular, and branched structures for the photocatalytic inactivation of Escherichia coli (E. coli) in water. Effects of film morphology and external applied voltage on inactivation rate were investigated. As-prepared films were characterized using s...

The aim of this study is to verify the reciprocity law in wastewater disinfection process using UV light. optical power UV-LEDs used were 1.6 mW and 50 mW, wavelengths 265 nm 275 nm. E. coli, Enterococcus faecalis, Clostridium perfringens three microorganisms analysed study. results showed lower inactivation rates around 0.063–0.065 cm2/mJ for 0.047–0.049 compared with other two bacteria. For c...

BACKGROUND: Ultraviolet reactors that are used for drinking water disinfection need to be tested to ensure they are delivering the required doses and achieving the desired level of pathogen inactivation to protect public health. Testing methods can use biological, chemical, or particle surrogates, although not all approaches have been approved for reactor validation. The non-pathogenic bacterio...

When exposed to 254-nm UV, spores of Encephalitozoon intestinalis, Encephalitozoon cuniculi, and Encephalitozoon hellem exhibited 3.2-log reductions in viability at UV fluences of 60, 140, and 190 J/m(2), respectively, and demonstrated UV inactivation kinetics similar to those observed for endospores of DNA repair-defective mutant Bacillus subtilis strains used as biodosimetry surrogates. The r...

Various lines of evidence have shown that ALDH3A1 (aldehyde dehydrogenase 3A1) plays a critical and multifaceted role in protecting the cornea from UV-induced oxidative stress. ALDH3A1 is a corneal crystallin, which is defined as a protein recruited into the cornea for structural purposes without losing its primary function (i.e. metabolism). Although the primary role of ALDH3A1 in the metaboli...

Because Cryptosporidium parvum oocysts are very resistant to conventional water treatment processes, including chemical disinfection, we determined the kinetics and extent of their inactivation by monochromatic, low-pressure (LP), mercury vapor lamp UV radiation and their subsequent potential for DNA repair of UV damage. A UV collimated-beam apparatus was used to expose suspensions of purified ...

We studied inactivation and UV disinfection of murine norovirus (MNV) as a surrogate for human norovirus. We investigated the effects of different surface characteristics, temperatures, and NaCl concentrations on MNV survival using both a plaque assay and a real-time TaqMan reverse transcription (RT)-PCR assay. MNV survived more than 40 days on diaper material, on gauze, and in a stool suspensi...

We have developed a host cell reactivation assay of DNA repair utilizing UV-treated plasmid vectors. The assay primarily reflects cellular repair of transcriptional activity of damaged DNA measured indirectly as enzyme activity of the transfected genes. We studied three plasmids (pSV2cat, 5020 base pairs; pSV2catSVgpt, 7268 base pairs; and pRSVcat, 5027 base pairs) with different sizes and prom...

The paper considers the combined effect of ultraviolet radiation (UV) and ultrasonic cavitation (ultrasonic) as part filtration systems on representatives saprophytic conditionally pathogenic microflora water. Ultrasound examination (25 kHz) is a promising method purification in aquaculture due to possibility inactivation microorganisms agglomerates that are not exposed UV. As result study cult...