The hen oviduct has been observed to lose virtually all its ability to incorporate free ammo acid into protein upon homogenation (2). In many instances, however, homogenation of a prelabeled mince, followed by removal of most of the radioactivity, and subsequent incubation, has led to a larger quantity of radioactivity contained in the protein fraction than was present at the start or early in ...

diet furnishing daily requirement of valine for optimal growth (70 iumol/g) and all other nutrients ("complete diet"), they gain weight at an average rate of 3.0 g/100 g body wt/day. When valine is removed, they lose weight at an average 2.1 g/100 g body wt/day. The growth retardation is improved or corrected by adding valine to the diet, daily weight gain being proportional to dietary valine c...

The beta-lactam antibiotic penicillin is produced as an end product by some filamentous fungi only. It is synthesized from the amino acid precursors L-alpha-aminoadipic acid, L-cysteine, and L-valine. Previous data suggested that certain amino acids play a role in the regulation of its biosynthesis. Therefore, in this study the effects of externally added amino acids on both Aspergillus (Emeric...

Glycerol-stabilised cell extracts of Streptomyces clavuligerus contain an enzyme activity which synthesises ACV from the individual amino acids L-a-aminoadipic acid, t-cysteine and L-valine. Enzyme activity was optimum in reaction mixtures containing 1 mM ATP together with an ATP regenerating system. The ACV synthetase enzyme formed ACV analogs when provided with Lcarboxymethylcysteine in place...

Four experimental diets for newly weaned pigs were formulated: (1) low valine and low tryptophan; (2) low valine and high tryptophan; (3) high valine and low tryptophan and (4) high valine and high tryptophan. Dietary standardized ileal digestible (SID) lysine content was 1.06 g/kg. The SID valine to SID lysine ratio was 0.58 and 0.67 for the low and high valine diets, respectively, and SID try...

The reaction of two D-amino acid oxidases from the yeasts Rhodotorula gracilis and Trigonopsis variabilis with the substrates alanine and valine in their 2-1H and 2-2H forms was studied employing the stopped-flow spectrophotometric technique. The turnover numbers at infinite substrate and oxygen concentrations were: 20,700/4,250 and 1,730/360 ([2-1H]/[2-2H]alanine and valine, respectively) for ...

BACKGROUND Advanced glycation end-products (AGEs) contribute to the pathogenesis of diabetic glomerulopathy. The role of AGEs in non-diabetic renal damage is not well characterized. First, we studied whether renal AGE accumulation occurs in non-diabetic proteinuria-induced renal damage and whether this is ameliorated by renoprotective treatment. Secondly, we investigated whether renal AGE accum...

Sows (98 first parity and 104 second parity) were used to determine the effects of dietary lysine and valine on lactation performance. Treatments were arranged in a 2 x 3 factorial with two levels of lysine (.80 or 1.20%) and three valine:lysine ratios (80, 100, or 120% of lysine). For all sows, increasing dietary lysine increased litter weaning weight (P < .001) and litter weight gain (P < .00...

An autoradiographic method for the measurement of the rate of valine incorporation into brain proteins is described. The transfer coefficients for valine into and out of the brain and the rate of valine incorporation into normal rat brain proteins are given. The valine incorporation and the transfer constants of valine between different biological compartments are provided for 14 gray matter an...