Vesicular stomatitis virus (VSV) is an emergent affecting livestock in the US. Previously, using a recombinant VSV carrying M51R mutation matrix protein (rNJ0612NME6-M51R), we evaluated pathogenesis of this pigs. Our results indicated that rNJ0612NME6-M51R represented attenuated phenotype in-vivo and ex-vivo pig macrophages, resembling certain clinical features observed field isolates. In order...

In this contribution, we provide an overview of gold compound applications against viruses or parasites during recent years. The special properties have been the subject intense investigation in years, which has led to development its chemistry with synthesis new compounds and study applicability various areas such as catalysis, materials, nanotechnology medicine. Herein, thirteen articles seve...

Understanding the efficacy and durability of heterologous immunization schedules against SARS-CoV-2 is critical, as supply demands vaccine choices become significant issues in global vaccination strategy. Here we characterize neutralizing antibodies produced two subjects who received combination immunizations SARS-CoV-2, first with Covishield (Oxford–AstraZeneca) vaccine, followed 33 days later...

The effect of low pH's on the development of vesicular stomatitis virus (VSV) was studied. L cells infected with VSV were incubated at pH 6.6. A 99% inhibition in the yield of infectious particles was observed by comparison with the yield at pH 7.4. Such inhibition was not due to inhibition of viral RNA synthesis, since at pH 6.6 all the known species of VSV RNA molecules were synthesized. Furt...

Sigmodon hispidus (cotton rat) was previously found to have antibodies against Vesicular Stomatitis Virus, New Jersey serotype (VSV-NJ) under field conditions in Costa Rica. In order to evaluate their response to the virus and potential role as reservoirs, a total of 30 adult laboratory-raised cotton rats were experimentally inoculated with 107 TCID50/ml VSV-NJ, Greentree strain, as follows: 10...

Vesicular stomatitis virus (VSV) is highly immunogenic and able to stimulate both innate and adaptive immune responses. However, its ability to induce adverse effects has held back the use of VSV as a potential vaccine vector. In this study we developed VSV-ΔP, a safe yet potent replication-defective recombinant VSV in which the phosphoprotein (P) gene was deleted. VSV-ΔP replicated only in sup...

The envelope glycoprotein of vesicular stomatitis virus (VSV-G) enables viral entry into hosts as distant as insects and vertebrates. Because of its ability to support infection of most, if not all, human cell types VSV-G is used in viral vectors for gene therapy. However, neither the receptor nor any specific host factor for VSV-G has been identified. Here we demonstrate that infection with VS...

It is essential for the study of T-cell function and the improvement of adoptive cell therapies to efficiently generate large populations of human primary T cells that reliably express foreign genes. This goal is achieved by using recombinant retroviruses pseudotyped with either the gibbon ape leukemia virus (GaLV) envelope or the vesicular stomatitis virus G (VSV-G) glycoprotein. We show here ...

We have isolated a vesicular stomatitis virus (VSV)-specific CD4+ CD8- murine T-cell clone. This clone proliferates only in response to VSV and lyses infected tumor cells bearing class II major histocompatibility antigens in short-term chromium release assays. In addition, the cell has VSV antigens on its surface and is capable of killing uninfected tumor cells without major histocompatibility ...

Using monoclonal antibodies and indirect immunofluorescence microscopy, we investigated the distribution of the M protein in situ in vesicular stomatitis virus-(VSV) infected MDCK cells. M protein was observed free in the cytoplasm and associated with the plasma membrane. Using the ts045 mutant of VSV to uncouple the synthesis and transport of the VSV G protein we demonstrated that this distrib...