RNA silencing is a mechanism which higher plants and animals have evolved to defend against viral infection in addition to regulation of gene expression for growth and development. As a counterdefense, many plant and some animal viruses studied to date encode RNA silencing suppressors (RSS) that interfere with various steps of the silencing pathway. In this study, we report the first identifica...

background: rna interference (rnai) is the mechanism of gene silencing-mediated messenger rna degradation by small interference rna (sirna), which becomes a powerful tool for in vivo research, especially in the areas of cancer. in this research, the potential use of an expression vector as a specific sirna producing tool for silencing of bcr-abl in k562 cell line has been investigated. methods:...

Recent advances in potexvirus research have produced new models describing virus replication, cell-to-cell movement, encapsidation, R gene-mediated resistance and gene silencing. Interactions between distant RNA elements are a central theme in potexvirus replication. The 5' non-translated region (NTR) regulates genomic and subgenomic RNA synthesis and encapsidation, as well as virus plasmodesma...

Both the biotic and abiotic stressors restrict yield potential of many crops, including bananas. Bananas belong to genus Musa are world’s most popular widely produced fruit for their nutritional industrial importance. The demand bananas is growing each day worldwide. However, different pest infestations hampering production bananas, making it a matter concern global food security. Several biote...

The Agrobacterium-mediated transient expression assay in intact tissues has emerged as a rapid and useful method to analyze genes and gene products in plants. In many cases, high levels of active protein can be produced without the need to produce transgenic plants. In this study, a series of tools were developed to enable strong or weak induction of RNA silencing and to suppress RNA silencing ...

Bacterial canker of tomato is a systemic disease caused by Clavibacter michiganensis (Cm), which poses grave threat to production worldwide. Towards the identification genes underlying resistance Cm infection, transcriptome resistant inbred backcross line IBL2353 carrying Rcm2.0 locus derived from Solanum habrochaites LA407 and susceptible lycopersicum Ohio88119 was comparatively analyzed after...

Small interfering RNA (siRNA) induced posttranscriptional gene silencing (PTGS) has been an efficient method for genetic and molecular analysis of certain developmental and physiological processes and represented a potential strategy for both controlling virus replication and developing therapeutic products. However, there are limitations for the methods currently used to deliver siRNA into cel...