Taste sensory responses from the chorda tympani nerve of the rat were recorded with the lingual receptive field under current or voltage clamp. Consistent with previous results (Ye, Q., G. L. Heck, and J. A. DeSimone. 1993. Journal of Neurophysiology. 70:167-178), responses to NaCl were highly sensitive to lingual voltage clamp condition. This can be attributed to changes in the electrochemical...

Subthreshold potentials are thought to be mediated by time-independent, "passive" background currents. In this study, we show that the background current-voltage (I-V) relation of guinea pig ventricular myocytes is changed significantly by repetitive stimulation, in such a way that cell excitability becomes enhanced. Myocytes were used for whole-cell voltage-clamp experiments. A voltage-clamp r...

Patch-clamp recording techniques have revolutionized understanding of the function and sub-cellular location of ion channels in excitable cells. The cell-attached patch-clamp configuration represents the method of choice to describe the endogenous properties of voltage-activated ion channels in the axonal, somatic and dendritic membrane of neurons, without disturbance of the intracellular milie...

,4BSTRACT TASAKI, I. AND C. S. SPYROPOULOS. Membrane conductance and currentvoltage relation in the squid axon under ‘voltage-clamp.’ &L-The conductance of the squid axon membrane under ‘voltage-clamp’ was measured by superposing a sinusoidal wave upon rectangular clamping voltage pulses. It was possible to determine the time course of the emf of the membrane under ‘voltage-clamp’ on a single p...

The current understanding of Ca(2+) channel function is derived from the use of the patch-clamp technique. In particular, the measurement of fast cellular Ca(2+) currents is routinely achieved using whole-cell voltage-clamp recordings. However, this experimental approach is not applicable to the study of local native Ca(2+) channels during physiological changes of membrane potential in complex ...

Voltage-gated ion channels mediate electrical dynamics in excitable tissues and are an important class of drug targets. Channels can gate in sub-millisecond timescales, show complex manifolds of conformational states, and often show state-dependent pharmacology. Mechanistic studies of ion channels typically involve sophisticated voltage-clamp protocols applied through manual or automated electr...

Effects of the antiepileptic drug valproate on sodium and potassium currents in the nodal membrane of peripheral nerve fibers of Xenopus laevis were determined by voltage- and current-clamp experiments. Under voltage-clamp conditions, a reduction of both sodium and potassium conductance (in a ratio of 2:1) was observed. Typically, 2.4 mM (400 mg/L) valproate reduced the sodium current 54% and t...

FORWARD CONVERTER Jhao-Cyuan Huang, Yong-seng Wong, Kuo-Bin Liu, NSRRC, Hsinchu, Taiwan Abstract The traditional pulse-width-modulated flyback converter power switch has serious electromagnetic interference (EMI) and lower conversion efficiencies problems due to the hard-switching operations. This paper produces a zero voltage switching of flyback-forward converter with an active-clamp circuit,...

This paper discusses the design, fabrication, and test of a CMOS active clamp circuit. The active clamp is a linear voltage regulator, with a voltage deadband to allow for voltage ripple, that is designed to operate in parallel with a switchmode voltage regulator. Its specific function is to sink or source large transient currents to microprocessor loads, thus allowing operation with very small...