Recently it has been observed that liver enzyme activity may be markedly affected by dietary conditions. Seifter et al. (1) have demonstrated that during complete dietary protein deprivation loss of arginase and n-amino acid oxidase from rat liver occurs more rapidly than loss of general liver protein. We (2) have observed that the level of xanthine oxidase activity in rat liver may be altered ...

The HPTLC analysis, antioxidant, and antigout activity of Asparagus racemosus, Withania somnifera, Vitex negundo, Plumbago zeylanica, Butea monosperma and Tephrosia purpurea extracts were investigated. The chemical fingerprinting were carried out by high performance thin layer chromatography (HPTLC), antioxidant activity by ABTS, DPPH, FRAP radical scavenging assays, and antiogout activity by c...

Treatment of milk xanthine oxidase and chicken liver xanthine dehydrogenase with high concentrations of salts for several hours resulted in the loss of reactivity toward O2 and NAD, respectively. Oxidation of xanthine by either enzyme in the presence of electron acceptors such as dichlorophenol indophenol was unimpaired under these conditions, in the presence of 3 M KI. Prolonged treatment of x...

Biogenesis of prokaryotic molybdoenzymes is a complex process with the final step representing the insertion of a matured molybdenum cofactor (Moco) into a folded apoenzyme. Usually, specific chaperones of the XdhC family are required for the maturation of molybdoenzymes of the xanthine oxidase family in bacteria. Enzymes of the xanthine oxidase family are characterized to contain an equatorial...

The twin character of reactive oxygen species is substantiated by a growing body of evidence that reactive oxygen species within cells act as inducers and accelerators of the oncogenic phenotype of cancer cells, while reactive oxygen species can also induce cancer cell death and can therefore function as anti-tumorigenic species. The aim of this study was to assess a possible influence of xanth...

The composition of milk xanthine oxidase has been reinvestigated. When the enzyme is prepared by methods that include a selective denaturation step in the presence of sodium salicylate the product is obtained very conveniently and in high yield, and is homogeneous in the ultracentrifuge and in recycling gel filtration. It has specific activity higher than previously reported preparations of the...

BACKGROUND Increased inactivation of nitric oxide by superoxide (O2*-) contributes to endothelial dysfunction in patients with coronary disease (CAD). We therefore characterized the vascular activities of xanthine oxidase and NAD(P)H oxidase, 2 major O2*--producing enzyme systems, and their relationship with flow-dependent, endothelium-mediated vasodilation (FDD) in patients with CAD. METHODS...

Interferon, interferon inducers, and a variety of other immunomodulators are known to depress the hepatic cytochrome P-450 drug-metabolizing system. Two concepts have been proposed to explain this phenomenon. (a) The steady-state of cytochrome P-450 is altered through decreased synthesis and increased degradation of cytochrome P-450 apoprotein. (b) Interferon induces xanthine oxidase; superoxid...

The electron acceptor 2,6-dichlorophenolindophenol (DCI) forms a spectrophotometrically and kinetically detectable complex with bovine milk xanthine oxidase. The visible absorption spectrum of complexed DC1 exhibits an absorption maximum at 660 nm at pH 7.8 (E = 34.2 X lo3 M-’ cm-‘), a marked bathochromic shift from the visible absorption maximum at 608 nm characteristic of the free dye (E = 21...

A recent finding from this laboratory indicated that the incubation in vitro of purified xanthine oxidase with trace amounts of l-ascorbic acid resulted in a marked inhibition of the enzymatic activity (1, 2). Since the physiological mode’of action of Z-ascorbic acid is still obscure, it seemed desirable to determine whether ascorbic acid would, in the intact animal, exert a similar effect on x...