Recombinase encoded by the R gene of pSR1 of Zygosaccharomyces rouxii mediates reciprocal recombination between two specific recombination sites (RSs) to induce excision or inversion of the DNA segment that is flanked by the RSs. We report here that site-specific recombination mediated by this system takes place effeciently in tobacco cells. To monitor the recombination events in tobacco cells,...

We investigated the growth parameters of Saccharomyces rouxii isolated from spoiled chocolate syrup. The optimum pH range for S. rouxii was 3.5 to 5.5, whereas the minimum and maximum pH values that permitted growth were 1.5 and 10.5, respectively. For cells grown in 0 and 60% sucrose the optimum water activity (aw) values were 0.97 and 0.96, respectively. The optimum temperature for S. rouxii ...

Osmophilic yeasts have been studied largely with respect to their ability to grow in high concentrations of sugar but, until recently, very little attention has been paid to the kind and amount of products formed. Nickerson (1943) isolated a yeast from sour wine that he classified as Zygosaccharomyces acidifaciens and later, together with Carroll (Nickerson and Carroll, 1945), studied the produ...

食品由来の酵母19菌種75株についてポテトデキストロース寒天培地, 25 °C，5日間培養した後, VITEK MSTM plusを用いてマススペクトルを取得し, SuperSpectrum (SSp) を作成した. 作成したSSpについて検証を行った結果, 同定の信頼性レベルはSchizosaccharomyces 属, Sterigmatomyces elviae, Starmerella apicola, S. etchellsii, Wickerhamomyces subpelliculosus, Zygosaccharomyces rouxii，Zygosaccharomyces bisporus では80 %以上であった. Z. bailii，Z. parabailii，Z. pseudobailii，Z. mellis, およびZ. siamensisは, それぞれのS...

The Zygosaccharomyces rouxii plasmid pSB3 is one of the yeast plasmids resembling 2-microns DNA. The presence of a cis-acting locus needed for stable maintenance of pSB3 has been expected by analogy with the functions encoded by other yeast plasmids such as 2-microns DNA and pSR1. We found such a locus and designated it STB-SB3. The STB-SB3 locus was delimited within 371 bp in a unique region o...