Synthetic biology aspires to construct natural and non-natural pathways to useful compounds. However, pathways that rely on multiple promiscuous enzymes may branch, which might preclude selective production of the target compound. Here, we describe the assembly of a six-enzyme pathway in Escherichia coli for the synthesis of C50-astaxanthin, a non-natural purple carotenoid. We show that by judi...

Abstract. Data compiled from eight decades of incipient motion studies were used to calculate dimensionless critical shear stress values of the median grain size, t*c50. Calculated t*c50 values were stratified by initial motion definition, median grain size type (surface, subsurface, or laboratory mixture), relative roughness, and flow regime. A traditional Shields plot constructed from data th...

We present single-channel approaches to robust automatic speech recognition (ASR) in reverberant environments based on non-intrusive estimation of the clarity index (C50). Our best performing method includes the estimated value of C50 in the ASR feature vector and also uses C50 to select the most suitable ASR acoustic model according to the reverberation level. We evaluate our method on the REV...

UNLABELLED Haloarcula japonica, an extremely halophilic archaeon that requires high concentrations of NaCl for growth, accumulates the C50 carotenoid bacterioruberin (BR). By homology analysis, a gene cluster, including c0507, c0506, and c0505, was found and predicted to be involved in the synthesis of bacterioruberin. To elucidate the function of the encoded enzymes, we constructed Ha. japonic...