The expression of csdA, encoding an RNA helicase, was induced at 3°C in Yersinia pseudotuberculosis. The role of CsdA in Y. pseudotuberculosis under cold conditions was confirmed by impaired growth of insertional csdA mutants at 3°C. The results suggest that CsdA is crucial for Y. pseudotuberculosis survival in the chilled food chain.

CsdA, a DEAD-box protein from Escherichia coli, has been proposed to participate in a variety of processes, such as translation initiation, gene regulation after cold-shock, mRNA decay and biogenesis of the small ribosomal subunit. Whether the protein really plays a direct role in these multiple processes is however, not clear. Here, we show that CsdA is involved in the biogenesis of the large ...

Introduction Stopping media are characterized by their stopping power (SP), the inelastic mean free path (IMFP), the continuous slowing down approximation-range (CSDA-range) (R) and the energy straggling parameter. These physical quantities are important for application such as radiation biology, electron beam lithography, and chemical analyses of surface regions of a solid and in calculation o...

PURPOSE To study the energy deposition by low-energy electrons in submicron tissue-equivalent targets by comparing two widely used methodologies, namely, the continuous-slowing-down-approximation (CSDA) convolution integral and the Monte Carlo (MC) simulation. METHODS An MC track-structure code that simulates collision-by-collision the complete slowing down process is used to calculate the en...

Biogenesis of iron-sulfur (Fe-S) cluster-containing proteins relies on assistance of complex machineries. To date three systems, NIF, ISC, and SUF, were reported to allow maturation of Fe-S proteins. Here we report that the csdA-csdE (formally ygdK) genes of Escherichia coli constitute a sulfur-generating system referred to as CSD which also contributes to Fe-S biogenesis in vivo. This conclusi...

Microorganisms inhabiting Antarctic biocrusts develop several strategies to survive extreme environmental conditions such as severe cold and drought. However, the knowledge about adaptations of microorganisms are limited. Here, we applied metagenomic sequencing study from east Antarctica. Biocrusts were dominated by cyanobacteria, actinobacteria proteobacteria. Furthermore, results provided ins...

In Escherichia coli, the cold shock response occurs when there is a temperature downshift from 37 degrees C to 15 degrees C, and this response is characterized by induction of several cold shock proteins, including the DEAD-box helicase CsdA, during the acclimation phase. CsdA is involved in a variety of cellular processes. Our previous studies showed that the helicase activity of CsdA is criti...

CsdA is one of five E. coli DEAD-box helicases and as a cold-shock protein assists RNA structural remodeling at low temperatures. The helicase has been shown to catalyze duplex unwinding in an ATP-dependent way and accelerate annealing of complementary RNAs, but detailed kinetic analyses are missing. Therefore, we performed kinetic measurements using a coupled annealing and strand displacement ...

An efficient architecture of 1D CSDA MST core is designed using CSDA (Common Sharing Distributed Arithmetic) to achieve high-throughput rate supporting multistandard transformations at low cost. Common sharing distributed arithmetic (CSDA) combines factor sharing and distributed arithmetic sharing techniques, efficiently reducing the number of adders for high hardware-sharing capability. Conven...

An efficient architecture of 1D CSDA MST core is designed using CSDA (Common Sharing Distributed Arithmetic) to achieve highthroughput rate supporting multistandard transformations at low cost. Common sharing distributed arithmetic (CSDA) combines factor sharing and distributed arithmetic sharing techniques, efficiently reducing the number of adders for high hardware-sharing capability. Convent...