BACKGROUND AND PURPOSE Salmonella enterica is one of the most common enteric pathogens worldwide. Conventional methods of isolation of Salmonella strains take 4-7 days to complete, are laborious and require substantial manpower. We devised a polymerase chain reaction (PCR) method that simultaneously uses three pairs of specific primers to detect inv, spv, and via genes of Salmonella. METHODS ...

By using nested PCR based on the nucleotide sequence encoding the Vi antigen, the ViaB region of Salmonella typhi, it has been possible to detect Salmonella typhi at the single-cell level. Amplification with two primer sets, R1, which amplified 599 bp of DNA (nucleotides 745 to 1343), and R2, which amplified 307 bp (nucleotides 877 to 1183), was associated with highly specific results for S. ty...

Salmonella enterica serotype Typhi (S. Typhi) is a causative organism of typhoid fever. A number of Salmonella serovars express a capsular polysaccharide antigen known as Vi, the biosynthetic and export proteins of which are encoded within the viaB locus of Salmonella Pathogenicity Island -7 (SPI-7). SPI-7 is inserted between two partially duplicated copies of tRNA -pheU gene. We have investiga...

The synthesis and transportation proteins of the Vi capsular polysaccharide of Salmonella enterica serovar Typhi (serovar Typhi) are encoded by the viaB operon, which resides on a 134-kb pathogenicity island known as SPI-7. In recent years, Vi-negative strains of serovar Typhi have been reported in regions where typhoid fever is endemic. However, because Vi negativity can arise during in vitro ...

Ulcerative colitis (UC) is characterized by presence of ulcer in colon and bloody diarrhea. The present study explores the possibility of association between Salmonella and ulcerative colitis. The present study comprised 59 cases of UC, 28 of colon cancer (CC), 127 of irritable bowel syndrome (IBS), and 190 of healthy control. The serological study was done by Widal and Indirect Haemagglutinati...

Strains of Salmonella typhi isolated from the blood of patients with typhoid fever invariably express a capsular polysaccharide, termed the Vi antigen. Vi antigen expression is controlled by two separate chromosomal loci, viaA and viaB. The viaA locus is commonly found in enteric bacteria. In contrast, the viaB locus appears to be specific to Vi-expressing strains of Salmonella and Citrobacter....