A particle-bound enzyme from the fungus imperfectus Cryfitococcus taurentii var. flavescens (NRRL Y-1401) mediates transfer of mannosyl units from GDP-mannose to endogenous primer. The enzyme requires divalent cations for activity. The pH optimum is ‘7.5 and the apparent K, for GDP-mannose is 0.14 mbr. A polysaccharide fraction that is associated with the enzyme was found to be similar to a pol...

A particle-bound enzyme from the fungus imperfectus Cryfitococcus taurentii var. flavescens (NRRL Y-1401) mediates transfer of mannosyl units from GDP-mannose to endogenous primer. The enzyme requires divalent cations for activity. The pH optimum is ‘7.5 and the apparent K, for GDP-mannose is 0.14 mbr. A polysaccharide fraction that is associated with the enzyme was found to be similar to a pol...

Haemophilus parasuis strains isolated from the noses of apparently healthy animals and from animals with pathological conditions were examined for the presence of a capsule, for their ability to agglutinate in acriflavine or after boiling, and for their peptide profile after polyacrylamide gel electrophoresis (PAGE). The capsule was identified by precipitation against hexadecyl trimethylammoniu...

Hairs varying from 6 to I2 in. in length were obtained mostly from women and in some cases from Sikh male members of the department. The hairs selected, after brittle, coarse, or extremely thin hairs had been discarded, were cleaned with 2 per cent. cetavlon, washed in water, wrapped around rubber tubing about 3 in. long to minimize curling, put into a wide-mouthed bottle, and autoclaved for 20...

Nine preparations of six antiseptic substances were applied to the perineum of pregnant women at term in order to assess their efficacy in sterilizing the skin. The efficacy, in descending order of effectiveness, after three minutes' exposure, was p-chlor-m-xylenol in alcohol (surgical Dettol); chlorhexidine gluconate in detergent (Hibiscrub), followed by the aqueous preparations p-chlor-m-xyle...

Previous studies with 31 strains of Serratia marcescens, including 28 reference O-serotype strains, have indicated that 19 of them have an acidic polysaccharide which copurifies with lipopolysaccharide during phenol-water extraction. Polysaccharide in crude extracts from 18 of the 19 strains was precipitated with Cetavlon (hexadecyltrimethyl ammonium bromide), and capsules were demonstrated aro...

The patient's sister had left hospital a few months earlier after sustaining a scald to the scalp which had required skin grafting. A proprietary shampoo had been recommended by a local shopkeeper to keep the scalp clean. One evening, another of the patient's siblings, her 7-year-old brother, rubbed some of this shampoo in during a nappy change. The patient was unsettled overnight, and presente...

The cell surfaces of five enteropathogenic Escherichia coli serotypes (O111:H2; O111:H12; O125:H9; O119:H6; O26:H11) were assayed by chemical methods, lectin agglutination tests and spectroscopy associated to transmission electron microscopy. Results of lectin agglutination assays showed that all strains reacted with mannosebinding lectins. Strains belonging to serotype O125:H9 also agglutinate...

A selective medium containing 0.1 % cetrimide in nutrient agar has been used here during the past four years for the routine identification of Ps. pyocyanea in burns (Lowbury, 1951). This medium, a modification of those previously described by Harper and Cawston (1945) and by Hood (1948), has proved convenient and efficient. In October, 1953, the manufacturers announced the production of a modi...