The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and specificity; thus, many small medium laboratories use it to perform all kinds single nucleotide polymorphisms (SNPs) genotyping works, as a molecular biotechnology for disease-related analysis. However, lack ava...

Calreticulin (CALR) mutations are included in the WHO diagnostic criteria of Myeloproliferative Neoplasm (MPN). Presence CALR mutation, aids their diagnosis by excluding reactive causes. There several molecular techniques to detect mutations; however, these require advanced equipment and technical expertise. An in-house “polymerase chain reaction-restriction fragment length polymorphism (PCR- R...

Abstract: The non-tuberculous mycobacteria (NTM) are ‘atypical mycobacteria’ belonging to species other than those classified in the Mycobacterium tuberculosis complex. NTM have been reported cause cross-reactive immune responses that interfere with diagnosis of both livestock and wildlife considered as potential pathogens for animals humans. present study was conducted detection non- tuberculo...

Introduction: The most common cutaneous fungal infections are caused by dermatophyte fungi such as Microsporum, Trichophyton, and Epidermophyton. In this study, the epidemiologic trends predominant organisms causing dermatophytosis in Urmia, Northwest Iran, were identified.Aims objectives: To get better perception of distribution authors studied identification isolated dermatophytes from human ...

The present study aimed at comparing the trypanosome specific 18S-PCR-RFLP using samples stored either on Whatman filter papers (PCR-RFLP-fp) or in a commercial cell lysis and DNA protecting buffer (PCR-RFLP-pb) with the haematocrit centrifugation technique (HCT), a method widely used for the diagnosis of African Animal Trypanosomosis. Out of 411 head of cattle, 49 (11.92%) (CI=8.95-15.45) scor...

Callipyge (CLPG) is a candidate gene with significant effect on meat quality and growth in sheep. The aim of this study was to investigate the polymorphism CLPG Akkaraman breed. In study, DNAs obtained from blood samples 50 sheep used as material. CLPG/Faq1 examined all individuals using method PCR-RFLP. Only A allele AA genotype were detected after PCR-RFLP procedure. This first which investig...