objectives: human immunodeficiency virus type 1 is the causative agent of acquired immunodeficiency syndrome “aids” in human and demonstration of hiv-1 genome in samples is accepted as evidence of infection. transmission of infection during window period in blood transfusion settings is a world wide concern. also there is a need for a rapid, sensitive and accurate technique to detect hiv-1 infe...

<p>Deoxyribonucleic acid (DNA) sequencing is the process of locating sequence main chemical bases in DNA. Next-generation (NGS) state-of-the-art DNA technique. The NGS technique advanced biological science analyzing human due to its scalability, high throughput, and speed. Analyzing crucial determine ability a person develop certain diseases his respond medications. ChIP-sequencing method...

We report the replication technology of DNA chip using by sequence specific localization of nucleic acids via hybridization and electric transfer of the nucleic acids onto a new substrate without losing their array information. The denatured DNA fragments are first spotted and UV-cross-linked on a nylon membrane. The membrane is then immersed and hybridized in a DNA mixture solution that contai...

Transcriptional regulation is largely enacted by transcription factors (TFs) binding DNA. Large numbers of TF binding motifs have been revealed by ChIP-chip experiments followed by computational DNA motif discovery. However, the success of motif discovery algorithms has been limited when applied to sequences bound in vivo (such as those identified by ChIP-chip) because the observed TF-DNA inter...