The protein bodies in maize endosperm are the sites of zein deposition. They are single membrane-bound vesicles with polysomes associated with the exterior surface of the membrane. These protein bodies were isolated by sucrose density gradients and characterized by electron microscopy and polyacrylamide gel electrophoresis. Polyribosomes dissociated from the surface of the membrane by detergent...

The interaction of 16 ring-substituted phenols and anilines with the corn protein zein was studied by reversed-phase high-performance liquid chromatography by preparing silica- and alumina-based stationary phases coated with various concentrations of zein. The relationship between the strength of interaction and the physicochemical parameters of solutes was elucidated by principal component ana...

Crystallins from pigeon eye lenses were isolated and purified by gel-permeation chromatography and characterized by gel electrophoresis, amino-acid composition and sequence analysis. Alpha- and beta-crystallins could be obtained in relatively pure forms by single-step size-exclusion chromatography whereas an extra step of ion-exchange chromatography was needed for the separation of delta-crysta...

HARI B. KRISHNAN,* MONTY S. KERLEY, GARY L. ALLEE, SUNGCHAN JANG, WON-SEOK KIM, AND CHUNJIANG J. FU U.S. Department of Agriculture-Agricultural Research Service, Plant Genetics Research Unit, 205 Curtis Hall, University of Missouri, Columbia, Missouri 65211, Division of Plant Science, 1-41 Agriculture Building, University of Missouri, Columbia, Missouri 65211, and Division of Animal Science, S1...

Zein is one of the seed storage proteins found in endosperm maize. It soluble aqueous ethanol solutions with high content, and forms aggregates when content solution lowered. This unique structural variation has long been utilized to prepare nanoparticles that carry hydrophobic drugs. In this research, optimal conditions for encapsulation menthol, major component peppermint oil, into zein nanoc...

Essential oils (EOs), which are derived from plants, have antifungal, insecticidal and antimicrobial activities, but they are slightly soluble in water and impart to the water their odor and taste, which limit their applications in food area. Zein, a prolamin from corn, is able to form nanoparticles by liquid-liquid dispersion process. These nanoparticles are well dispersed in water and stable,...

We investigated the effect of 3'-noncoding and poly(A) sequences on the translation and stability of membrane-bound mRNA (maize zein) and free mRNA (Xenopus beta-globin) by injecting SP6 transcripts into stage 6 Xenopus oocytes. With zein mRNA, the presence or absence of a 3'-noncoding or poly(A) sequence had little effect on mRNA stability over 24 h, and the 3'-noncoding sequence played essent...

Interactions of a model surfactant, sodium dodecyl sulfate (SDS), with a water-insoluble model protein, zein, were investigated to gain an understanding of the effects, such as skin irritation and protein denaturation, of surfactants that are common in personal-care products. To elucidate the mechanisms of such effects, the zeinprotein interactionwithSDSinaqueoussolutionswas investigatedusingam...

background and objectives: lauroyl arginine ethyl ester (lae) has been focused as a new active compound with antimicrobial property for food preservative. because of environmental concerns, the incorporation of preservatives into biodegradable films is more suitable than their incorporation into plastic films.the aim of this work was to develop antimicrobial including lae in bio zein matrix and...

Prolamins, the major cereal seed storage proteins, are sequestered and accumulated in the lumen of the endoplasmic reticulum (ER), and are directly assembled into protein bodies (PBs). The content and composition of prolamins are the key determinants for protein quality and texture-related traits of the grain. Concomitantly, the PB-inducing fusion system provides an efficient target to produce ...