Sex differentiation in fish is a highly labile process easily reversed by the use of exogenous hormonal treatment and has led to environmental concerns since low doses of estrogenic molecules can adversely impact fish reproduction. The goal of this study was to identify pathways altered by treatment with ethynylestradiol (EE2) in developing fish and to find new target genes to be tested further...

In this study the effect of Centella asiatica L. extract was studied against genotoxic doses of ethinylestradiol on human lymphocytes culture. The different doses of C. asiatica L. extract i.e. 1.075x10-4, 2.125x10-4 and 3.15x10-4 g/ml were treated separately with 5 μM of ethinylestradiol. Similar treatments were given with 10 μM of ethinylestradiol. The treatments result in a significant dose ...

In the present study the effect of Centella asiatica L. extract was studied against the genotoxic doses of ethinylestradiol on human lymphocytes culture. The different doses of C. asiatica L. extract i.e. 1.075×10 -4 , 2.125×10 -4 and 3.15×10 -4 g/ml were treated separately with 5 μM of ethinylestradiol. Similar treatments were given with 10 μM of ethinylestradiol. The treatments result in a si...

Abstract Effluents from municipal wastewater treatment plants (MWTPs) are complex mixtures of chemicals including endocrine-disrupting compounds (EDCs) and 17?-ethynylestradiol (EE2). The objective this study was to evaluate selected responses two fish species, in different years, exposed situ MWTP effluent. Biological markers exposure (plasma vitellogenin (VTG) antioxidant enzymes) were measur...

Adult ovariectomized Sprague-Dawley rats were administered a single initiating dose of 200 mg diethylnitrosamine (DEN)/kg, i.p. 17a-ethyn\ U-sirailiol (!•'.!•'...) was then chronically administered to the rats by means of s.c. Silastic implants at an estimated dose of 90 MgEE2/kg/day. Hepatic •Y-glutamyltranspeptidase-positivefoci were evaluated after 20-60 (+20,+30,...,+60) weeks of chro...

Hexane extract of the seeds of Nigella sativa L. prevented pregnancy in Sprague-Dawley rats treated orally at 2 g/kg daily dose on days 1-10 post-coitum. Significant antifertility activity was also observed in its column fractions and subfractions. At contraceptive dose, the active hexane extract exhibited only mild uterotrophic activity comparable almost to 0.002 mg/kg dose of 17 varies; is di...

BACKGROUND Environmental estrogens in wastewater treatment work (WwTW) effluents are well established as the principal cause of reproductive disruption in wild fish populations, but their possible role in the wider health effects of effluents has not been established. OBJECTIVES We assessed the contribution of estrogens to adverse health effects induced in a model fish species by exposure to ...

Sexual disruption is reported in wild fish populations living in freshwaters receiving discharges of wastewater treatment works (WwTW) effluents and is associated primarily with the feminisation of males by exposure to oestrogenic chemicals. Antiandrogens could also contribute to the feminisation of male fish, but there are far less data supporting this hypothesis and almost nothing is known fo...