Eight strains of Saccharomycopsis species were examined for their partial base sequences of 18S and 26S rRNAs. The base sequences on the fingerprint segment showed three kinds of ACUAU, UCUAU, and AUAU. In the partial base sequences (positions 1451-1618, 168 bases) of 18S rRNA, all the strains of Saccharomycopsis species examined had 1-0 base difference except for the strains of Saccharomycopsi...

Mutations at position C1054 of 16S rRNA have previously been shown to cause translational suppression in Escherichia coli. To examine the effects of similar mutations in a eukaryote, all three possible base substitutions and a base deletion were generated at the position of Saccharomyces cerevisiae 18S rRNA corresponding to E. coli C1054. In yeast, as in E. coli, both C1054A (rdn-1A) and C1054G...

Plasmid pBG 35 contains the whole rDNA unit from flax (Linum usitatissimum L.) (Goldsbrough and Cullis, 1981). We cloned four different Eco RI restriction rDNA fragments of pBG 35 in the plasmid vector pBluescript SK+ (pBS). One of the subclones (p2.1) contained the most part of the 5 ́-end of 18S rRNA gene (1581 bp) and was used as a heterologous DNA probe in molecular hybridization experiments...

Re-examination of the holotype of Burmoniscus kitadaitoensis Nunomura, 2009 from Kitadaitojima Island, southern Japan reveals that this species is a junior synonym of B. meeusei (Holthuis, 1947). Partial regions of mitochondrial COI, 12S and 16S rRNA genes, and nuclear 18S and 28S rRNA genes were detected for species identification in the future.

Molecular tools have revolutionized the exploration of biodiversity, especially in organisms for which traditional taxonomy is difficult (Tang et al., 2012). The small subunit (SSU) 18S rRNA gene is one of the most frequently used genes in phylogenetic studies and an important marker for random target PCR in environmental biodiversity screening. 18S rDNA is a nuclear ribosomal gene, commonly re...

Previous analyses of complexes of 40S ribosomal subunits with the hepatitis C virus (HCV) internal ribosome entry site (IRES) have revealed contacts made by the IRES with ribosomal proteins. Here, using chemical probing, we show that the HCV IRES also contacts the backbone and bases of the CCC triplet in the 18S ribosomal RNA (rRNA) expansion segment 7. These contacts presumably provide interpl...

In quantitative RT-PCR (qRT-PCR), analysis of gene expression is dependent on normalization using housekeeping genes such as 18S rRNA, GAPDH and beta actin. However, variability in their expression has been reported to be caused by factors like drug treatment, pathological states and cell-cycle phase. An emerging area of cancer research focuses on identifying the role of epigenetic alterations ...

We have determined the nucleotide sequence of part of a cloned ribosomal transcription unit from Xenopus laevis extending from the 3' region of the 18S gene through the 18S-28S intergene region into the start of the 28S gene. The 18S 3' region possess two tracts of high homology with the corresponding segments of other eukaryotic 18S genes (yeast and Bombyx mori) separated by a tract of low hom...

The sequences of 18S and 28S rDNAs have been used as molecular markers to resolve phylogenetic relationships of Heteroptera for two decades. The complete sequences of 18S rDNAs have been used in many studies, while in most studies only partial sequences of 28S rDNAs have been used due to technical difficulties of amplifying the complete lengths. In this study, we amplified the complete 18S and ...