On starch-gel or polyacrylamide-gel electrophoresis of human serum, a supernumerary zone of aspartate aminotransferase activity may be demonstrated, migrating with the slow alpha(2) protein zone. This appearance is due only to cationic aspartate aminotransferase, bound by alpha(2)-macroglobulin. The binding is strongly potentiated by dilute borate buffers.

Methods for the separation and identification of the polypeptide chains of each lens crystaUin are basic to the study of these proteins on polyribosomes. Rabbit lens proteins have been deaggregated, denatured, and reduced to polypeptide chains, and separated by polyacrylamide gel electrophoresis. Approximate molecular weights of the resultant chains have been calculated by the same system. Vigo...

Introduction 2 Deoxyribonucleic Acid to Ribonucleic Acid 4 Ribonucleic Acid to Protein 6 Proteome Handling in Classical and Functional Proteomics 6 Protein Purification 6 Cell Culture 8 Other Source of Samples 8 Subpopulation of Cells 10 Subcellular Components 11 Protein Separation by Two-Dimensional Gel Electrophoresis 12 Principles of Two-Dimensional Gel Electrophoretic Separation 13 Detectio...

The most common technique for sorting, identifying, and purifying nucleic acid fragments is agarose gel electrophoresis. performance of the electrophoresis could be impacted by buffers’ composition, ionic strength, pH characteristics [1].

We studied serum proteomic profiling in patients with graft versus host disease (GVHD) after allogeneic hematopoietic cell transplantation (allo-HCT) by two-dimensional gel electrophoresis (2-DE) and mass spectrometry analysis. The expression of a group of proteins, haptoglobin (Hp), alpha-1-antitrypsin, apolipoprotein A-IV, serum paraoxonase and Zn-alpha-glycoprotein were increased and the pro...

A kininogenin (EC 3.4.21.8) was purified from the venom of Vipera ammodytes ammodytes (European sand viper) by a combination of gel filtration and ion-exchange chromatography. The enzyme is approximately six times more active than bovine trypsin in its ability to release vasoactive peptides from a plasma precursor. The kininogenin is a glycoprotein containing 18-20% by weight of carbohydrate. I...

A simple modification of the conventional one-dimensional sodium dodecyl sulfate--polyacrylamide gel electrophoresis technique has been used to visualize inter- and intramolecular disulfide bonding in proteins. The gradient of reducing agent established between adjacent slab gel tracks by electrophoresing identical protein samples next to one another, one containing and the other not containing...

The following observations indicate that cultured human WI-38 fibroblasts synthesize and secrete alpha2-macroglobulin into serum-free medium: (a) after incubation of cultures with [35S]L-methionine, a labeled protein appeared in the medium which was precipitated by antiserum directed against alpha2-macroglobulin; (b) after incubation of cultures with [35S]L-methionine, a major band of radioacti...

Human serum proteins were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis after protein denaturation in the presence or absence of 2-mercaptoethanol. Both electrophoretic techniques give characteristic and reproducible banding patterns and achieve a high degree of resolution within the limits of a one-dimensional separation. The major protein bands have been identified, a...