We used a baculovirus-based system to prepare structural proteins of hepatitis C virus (HCV) genotype 1a. Binding of this preparation to cultured human hepatic cells was both dose dependent and saturable. This binding was decreased by calcium depletion and was partially prevented by ligands of the asialoglycoprotein receptor (ASGP-R), thyroglobulin, asialothyroglobulin, and antibody against a p...

OBJECTIVE We identified lipocalin 2 (Lcn2) as a gene induced by dexamethasone and tumor necrosis factor-alpha in cultured adipocytes. The purpose of this study was to determine how expression of Lcn2 is regulated in fat cells and to ascertain whether Lcn2 could be involved in metabolic dysregulation associated with obesity. RESEARCH DESIGN AND METHODS We examined Lcn2 expression in murine tis...

An indole alkaloid tumor promoter, dihydroteleocidin B, was able to modulate a membrane property of 3T3-L1 preadipocytes, showing an almost complete reduction of epidermal growth factor binding capacity. This receptor modulating potency of dihydroteleocidin B, was 10 times that of a phorbol ester tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Dihydro teleocidin B, however, had littl...

An indole alkaloid tumor promoter, dihydroteleocidin B, was able to modulate a membrane property of 3T3-L1 preadipocytes, showing an almost complete reduction of epidermal growth factor binding capacity. This receptor modulating potency of dihydroteleocidin B, was 10 times that of a phorbol ester tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). Dihydroteleocidin B, however, had little...

Overexpression of Myc in cells can suppress the transcription of specific genes. Because several of these genes have common transcriptional regulatory elements, we investigated the possibility that this effect of Myc is mediated through a specific transcription factor. In vitro DNA-binding assays detect only one form of CCAAT transcription factor/nuclear factor 1 (CTF/NF-1) in quiescent 3T3-L1 ...