C35 was reported to be a new biomarker and therapeutic target for breast cancer. To explore the functional importance of C35, we constructed small interfering RNA (siRNA) targeting C35 and investigated the effects of the siRNAs on C35 expression and apoptosis of T47D cells. C35 siRNAs were constructed and named psiRNA-C35-1 and psiRNA-C35-2. Reverse transcription-polymerase chain reaction (RT-P...

The aim of this study was to investigate the expression of C35, an oncogene previously found in breast and prostate cancers, and its clinicopathological significance in colorectal cancer (CRC). Qualitative and quantitative detection of C35 mRNA expression was performed using reverse transcription-PCR (RT-PCR) and real-time PCR. C35 protein expression was determined using immunohistochemistry. C...

BACKGROUND Resistance to human epidermal growth factor receptor-2 (HER2)-targeted therapies is common, and results in treatment failure and new tumor progression. C35 is over-expressed in many invasive breast cancer endogenously, and functions as an oncogene in breast cancer cell lines. METHODS The current study aims to investigate effects of C35 on cell viability and migration of HER2-positi...

C35 is a novel tumor biomarker associated with metastasis progression. To investigate the interaction factors of C35 in its high expressed breast cancer cell lines, we constructed bait recombinant plasmids of C35 gene and T47D cell cDNA library for yeast two-hybrid screening. Full length C35 sequences were subcloned using RT-PCR from cDNA template extracted from T47D cells. Based on functional ...

I. The apparent digestibilities for chicks, operated on so as to allow separate collection of urine and faeces, of the nitrogen in a heat-damaged cod flour (C35) and of a control, freezedried cod muscle (Cz3) were 77 and 90 yo respectively. 2. The differences are similar to those found for rats in earlier work and considerably smaller than the differences found in nutritional value of the mater...

Upon coexpression with Erwinia geranylgeranyldiphosphate (GGDP) synthase in Escherichia coli, C(30) carotenoid synthase CrtM from Staphylococcus aureus produces novel carotenoids with the asymmetrical C(35) backbone. The products of condensation of farnesyldiphosphate and GDP, C(35) structures comprise 40 to 60% of total carotenoid accumulated. Carotene desaturases and carotene cyclases from C(...

The aim of this study was to show the possibility of the classification of structural timber (European spruce) into strength class C35 according to EN 338 by using combined visual and machine strength grading techniques. Three different visual machine strength grading combinations are devised for allocating sawn timber into strength class C35. They are composed on the one hand of the visual gra...

Heptaprenyl pyrophosphate synthetase was detected in partially purified extracts of Bacillus subtilis. The enzyme catalyzed the synthesis of all-trans C35 prenyl pyrophosphate from isopentenyl pyrophosphate and farnesyl or geranylgeranyl pyrophosphate, but it did not catalyze a reaction between isopentenyl pyrophosphate and either dimethylallyl or geranyl pyrophosphate. The enzyme reaction proc...

in this research, catalase activity and leaf chlorophyll stability of satsuma mandarin on some citrus rootstocks under waterlogging condition were studied. experiment was conducted as factorial with randomized complete blocks and 6 replications. the first factor, include 7 soils from east mazandaran and the second factor included 7 different citrus rootstocks: 1. sour orange, 2. swingle citrome...