the objective of this study was to estimate the effective number of breeders in sarabi cattle population using heterozygote-excess method based on single nucleotide polymorphism markers. data consisted of 20 sarabi cows. snp genotyping was performed using illumina high-density bovine beadchip designed to genotype 777,962 snps. average observed heterozygosity, expected heterozygosity, minor alle...

The melanocortin-1 receptor (MC1R) gene has been investigated by many studies regarding the pigmentation variation in various species. In order to determine its allelic and genotypic distribution, we sequenced the goat MC1R gene from 78 individuals in ten populations (Gembrong, Senduro, Ettawa Grade, Boerawa, Boerka, Kosta, Samosir, Muara, Boer and Kacang). Direct sequencing m...

Wuyi Mountain in Southeast China is the origin of black tea and oolong tea. It also considered ‘treasure trove cultivars’ because its rich germplasm resources. In present study, population structure genetic diversity 137 germplasms from adjacent areas were analyzed by SNPs. The information index (I), observed heterozygosity (Ho), expected (He) fixation (F) polymorphisms selected SNPs high, stab...

Most previous studies on genetic fingerprinting and cultivar relatedness in sweet cherry were based on isoenzyme, RAPD, and simple sequence repeat (SSR) markers. This study was carried out to assess the utility of single nucleotide polymorphism (SNP) markers generated from 3' untranslated regions (UTR) for genetic fingerprinting in sweet cherry. A total of 114 sweet cherry germplasm representin...

Hynobius amjiensis was considered as one of the 29 most threatened amphibian in China. To effectively conserve, manage and recover population this critically endangered species, 33 single nucleotide polymorphism (SNP) markers were developed using double digest restriction-site associated DNA (ddRAD) sequencing. The minor allele frequency per locus ranged from 0.0161 to 0.5000. observed heterozy...

This study attempted to infer the population structure of Japanese Black cattle by using genotype data on 33,063 genomewide single nucleotide polymorphism (SNP) markers totally 4,348 fattened steers slaughtered at carcass markets in Tokyo, Osaka, Hyogo, Tottori, and Hiroshima prefectures. We evaluated allele frequency, heterozygosity, linkage disequilibrium, correlation phase, concordance among...

High-throughput genotyping methods have increased the analytical power to study complex traits but high cost has remained a barrier for large scale use in animal improvement. We have adapted genotyping-by-sequencing (GBS) used in plants for genotyping 47 animals representing 7 taurine and indicine breeds of cattle from the US and Africa. Genomic DNA was digested with different enzymes, ligated ...

The whole genome resequencing was used to develop single nucleotide polymorphisms (SNP) markers for the yellow catfish (Tachysurus fulvidraco). A total of 46 SNP from 129 individuals were selected 5550676 genotyping which distributed on 26 chromosomes. Of SNPs analyzed, 35 conformed Hardy-Weinberg equilibrium. observed and expected heterozygosity these ranged 0.2519 0.771 0.265 0.5018, respecti...

background: methylenetetrahydrofolate reductase (mthfr) single-nucleotide polymorphisms (snps) c677t and a1298c have been described as strong risk factors for idiopathic recurrent miscarriage (rm). however, very few studies have investigated the association of paternal mthfr snps with rm. the aim of the present study was to evaluate the prevalence of paternal c677t and a1298c snps among iranian...

Transcriptome sequencing technology has been applied in the development and discovery of single nucleotide polymorphism (SNP) markers in fish. In this study, a panel of 120 expressed sequence tag (EST)-derived SNPs was selected by several selection filters from the resultant EST library of Odontobutis potamophila using Illumina Sequencing. In total, 37 SNPs from 120 putative SNPs were considere...