chronic obstructive pulmonary disease (copd) has been defined by the global initiative for chronic obstructive lung disease (gold), as a disease state characterized by airflow limitation which is not fully reversible. copd consists of emphysema which is the destruction and inflammation of the lung alveoli. adenosine deaminase (ada, e.c.3.5.4.4) converts adenosine to inosine. there are two isoen...

background and objective: brucellosis is a main transmittable zoonotic disease, which is endemic, and a common health burden in iran. adenosine deaminase (ada) is an essential enzyme which is involved in purine metabolism and its role in immune system is very important. the aim of this study was to determine serum changes of ada and c-reactive protein (crp) levels in patients with brucellosis. ...

background in spite of recent advents, and novel improvements in the diagnosis and treatment of pulmonary tuberculosis (tb), definitive diagnosis of pulmonary tb in children is problematic. therefore, measurement of the serum level of adenosine deaminase (ada) was later introduced as a helpful marker for the diagnosis of tb. objectives the purpose of this study was to determine the usefulness o...

we describe the development of enterovirus meningoencephalitis associated with increased adenosine deaminase in cerebrospinal fluid of a 12-year-old boy, a known case of hypogamaglobulinemia despite monthly replacement of ivig. the patient was referred to our center with fever, headache and vomiting for 10 days. csf analysis was compatible with aseptic meningoencephalitis but high csf protein (...

tuberculosis pericarditis as a potentially fatal complication of tuberculosis requires effective diagnosis and treatment. we evaluated the efficacy of interferon-gamma (ifn-gamma) and adenosine deaminase (ada) for diagnosing tuberculosis pericarditis in a cohort of iranian patients presenting with pericarditis. we enrolled 38 patients with presentation of pericarditis. all patients underwent di...

The thermal denaturation of adenosine deaminase (ADA) has been investigated in the presence of sodium n-dodecyl sulphate (SDS) over the temperature range of (293-363K) in 2.5 mM phosphate buffer, pH 6.4 by temperature scanning spectroscopy. The interaction of SDS caused the folding of adenosine deaminanse resulting in a decrease of TH (temperature of minimum solubility), TS<...

the thermal denaturation of adenosine deaminase (ada) has been investigated in the presence of sodium n-dodecyl sulphate (sds) over the temperature range of (293-363k) in 2.5 mm phosphate buffer, ph 6.4 by temperature scanning spectroscopy. the interaction of sds caused the folding of adenosine deaminanse resulting in a decrease of th (temperature of minimum solubility), ts (temperature of maxi...

the effects of allopurinol, acyclovir and theophylline on the activity of adenosine deaminase (ada) were studied in 50 mm sodium phosphate buffer ph 7.5 at 27°c, using a uv– vis spectrophotometer. adenosine deaminase is inhibited by these ligands, via different types of inhibition. allopurinol, as a transition state analog of xanthine oxidase, and acyclovir competitively inhibit the catalytic a...

the effect of physiological concentrations of caffeine (purified from persian tea) on adenosine deaminase (ada) activity at physiological and pathological concentrations of adenosine (as substrate) in 50 mm tris-hcl buffer (ph 7.3) at 37°c was investigated, using uv-vis spectroscopy. ada exhibited a bi-phasic activity behavior and both phases showed positive cooperativities indicating adenosine...