background: differentiation, migratory properties and availability of mesenchymal stromal cells (msc) have become an important part of biomedical research. however, the functional heterogeneity of cells derived from different tissues has hampered providing definitive phenotypic markers for these cells. objective: to characterize and compare the phenotype and cytokines of adipose derived mscs (a...

objective: tendon never returns to its complete biological and mechanical properties after repair. bone marrow and, recently, adipose tissue have been used as sources of mesenchymal stem cells which have been proven to enhance tendon healing. in the present study, we compared the effects of allotransplantation of bone marrow derived mesenchymal stromal cells (bmscs) and adipose derived stromal ...

tendon never restores the complete biological and mechanical properties after healing. bone marrow and recently adipose tissue have been used as the sources of mesenchymal stem cells, which have been proven to enhance tendon healing. stromal vascular fraction (svf), derived from adipose tissue by an enzymatic digestion, represents an alternative source of multipotent cells, which undergo differ...

previous studies have demonstrated the potential of monotherapy with either mesenchymal stem cells (mscs) or estrogen in autoimmune and cuprizone models of multiple sclerosis (ms). the aim of this study was to examine the effects of co-administration of 17β-estradiol (e2) and adipose-derived mesenchymal stem cells (adscs) on remyelination of corpus callosum axons in a cuprizone model of ms. for...

conclusions large amounts of preclinical studies have been made of stem cells and there is an increasing interest to perform these studies within the human population but preclinical studies are insufficient; therefore, much more and efficient studies should be conducted to evaluate the efficacy and safety of stem cells. context musculoskeletal injuries may be painful, troublesome, life limitin...

Tendon never restores the complete biological and mechanical properties after healing. Bone marrow and recently adipose tissue have been used as the sources of mesenchymal stem cells, which have been proven to enhance tendon healing. Stromal vascular fraction (SVF), derived from adipose tissue by an enzymatic digestion, represents an alternative source of multipotent cells, which undergo differ...

conclusions according to the results of the present study, it can be concluded that adipose derived mscs can be cultured in serum-free media with no change in their differentiating capacity. this finding gives us a hope for future cell therapy studies and trials with little concern about zoonotic infections or immunological reaction. results the isolated cells showed adherent spindle shaped mor...

Human mesenchymal stem cells (hMSCs), which also called mesenchymal stromal cells, are multipotent stem cell. Human MSCs typically are positive for the surface markers CD44, CD73, CD90, CD105, CD106, and also negative for hematopoietic markers CD34 and CD45.These cells can be isolated from postnatal bone marrow, adipose tissue, placenta, and scalp tissue, as well as from various fetal tissues. ...

human mesenchymal stem cells (hmscs), which also called mesenchymal stromal cells, are multipotent stem cell. human mscs typically are positive for the surface markers cd44, cd73, cd90, cd105, cd106, and also negative for hematopoietic markers cd34 and cd45.these cells can be isolated from postnatal bone marrow, adipose tissue, placenta, and scalp tissue, as well as from various fetal tissues. ...

Citation: Gojanovich AD, Gimenez MC, Masone D, Rodriguez TM, Dewey RA, Delgui LR, Bustos DM and Uhart M (2018) Human Adipose-Derived Mesenchymal Stem/Stromal Cells Handling Protocols. Lipid Droplets and Proteins Double-Staining. Front. Cell Dev. Biol. 6:33. doi: 10.3389/fcell.2018.00033 Human Adipose-Derived Mesenchymal Stem/Stromal Cells Handling Protocols. Lipid Droplets and Proteins Double-S...