guish between apoptotic and necrotic cells, we performed an Annexin V assay with propidium iodide exclusion. In accordance with our TUNEL assay, .95% of the cells were Annexin V positive (unpublished results). Less than 5% of these Annexin V-positive cells showed simultaneous incorporation of propidium iodide. We therefore conclude that .95% of the plasmaderived cells are indeed in apoptosis an...

This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium Iodide solution followed by flow cytometry analysis. It is based on the principle that normal cells are hydrophobic in nature as they express phosphatidyl serine in the inner membrane (side facing the cytoplasm) and when the cells...

Evaluation of apoptosis by flow cytometry is generally accomplished by methods that use annexin V-FITC as vital dye, which access phosphatidylserine exposed on the external membrane at the beginning of this process. In addition, the concomitant use of propidium iodide makes possible to verify the characteristic nuclear alterations in the late stages of apoptosis, as a consequence of the increas...

Leukemia is a blood disease which is caused by the inhibition of differentiation and an increased proliferation rate. Nature has been known as a rich source of medically useful substances. High diversity of bioactive molecules extracted from marine invertebrates, makes them ideal candidates for cancer researches. The study has been done to investigate cytotoxic effects of dichloromethane brittl...

leukemia is a blood disease which is caused by the inhibition of differentiation and an increased proliferation rate. nature has been known as a rich source of medically useful substances. high diversity of bioactive molecules extracted from marine invertebrates, makes them ideal candidates for cancer researches. the study has been done to investigate cytotoxic effects of dichloromethane brittl...

Neutrophils play an important role in the defence of the bovine mammary gland against bacterial infections. In the course of the resolution of mammary gland inflammation, neutrophils undergo programmed cell death – apoptosis. The aim of this study was to confirm whether the co-cultivation of neutrophils of the bovine mammary gland with either Staphylococcus aureus or Streptococcus uberis leads ...

OBJECTIVE To quantitatively analyze apoptotic and secondary necrotic cells under apoptosis conditions. METHODS The cells of Burkitt lymphoma cell line Raji were incubated with 1.0 mumol/L dexamethasone (DEX) for 2, 4 and 8 h, then stained with Annexin V-FITC (fluorescein isothiocyanate conjugated) which was used to detect the exposure of phosphatidylserine (PS) on the out membrane resulting f...

Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM...