Kashmir is located in a region where winter temperatures are very low. During the winter months, the temperature in Kashmir is often below 0⁰ C. Generally concrete placed in colder regions with the help of insulated forms as well as heaters to protect freshly mixed concrete against freezing. These practices try to incorporate unnecessary carbon and leads to an disagreeable carbon footprint. The...

Antifreeze activity is induced by cold temperatures in winter rye (Secale cereale) leaves. The activity arises from six antifreeze proteins that accumulate in the apoplast of winter rye leaves during cold acclimation. The individual antifreeze proteins are similar to pathogenesis-related proteins, including glucanases, chitinases, and thaumatin-like proteins. The objective of this study was to ...

INTRODUCTION The aim of the study was to evaluate stability of 48 total parenteral admixtures for pediatric patients who require home parenteral nutrition. Admixtures contain high amounts of electrolytes. In a clinical practice electrolytes-enrichment of the parenteral nutrition admixtures is a usual demand, especially on the neonatal/pediatric wards. The supplementation of parenteral nutrition...

Antifreeze proteins are found in a wide range of overwintering plants where they inhibit the growth and recrystallization of ice that forms in intercellular spaces. Unlike antifreeze proteins found in fish and insects, plant antifreeze proteins have multiple, hydrophilic ice-binding domains. Surprisingly, antifreeze proteins from plants are homologous to pathogenesis-related proteins and also p...

Class I chitinases hydrolyse the β-1,4-linkage of chitin and also acquire antifreeze activity in some of the overwintering plants during cold stress. Two chitinases, HrCHT1a of 31 kDa and HrCHT1b of 34 kDa, were purified from cold acclimated and non-acclimated seabuckthorn seedlings using chitin affinity chromatography. 2-D gels of HrCHT1a and HrCHT1b showed single spots with pIs 7.0 and 4.6 re...

approved: Redacted foi Privacy Doyglass J. Stennett It has been standard practice in the United States to separate lipid emulsions from the other components of total parenteral nutrition due to the reported instability of admixed intravenous lipid emulsions. Some clinicians, however, have combined all TPN components into one container and administered these admixtures to patients without appare...

Injury inflicted by frost and freezing is found in all plants exposed to such damaging temperatures. Antifreeze proteins protect against these injurious temperatures by providing freeze resistance to organisms in which they occur. The expression of antifreeze proteins in a plant is a possible means of increasing the frost resistance and freeze tolerance of plants. Freeze tolerance curves of non...

An Arg-containing antifreeze glycoprotein from the polar fish Eleginus gracilis was isolated, and the major components were purified to homogeneity. The general protocol for purification was chromatography of serum on DEAE-cellulose, followed by chromatography on a cation exchanger. DEAE-cellulose chromatography resulted in two fractions, A and B. Fraction A contained most of the antifreeze gly...

The serum of winter flounder contains a group of antifreeze peptides which lower the freezing point of their body fluid in the winter. These antifreeze peptides disappear from their serum in the summer when the water temperature rises. Messenger RNA isolated from the liver of flounder collected in January directs the synthesis of predominantly one protein in an in vitro protein synthesis system...

The structure of the C-terminal antifreeze-like (AFL) domain of human sialic acid synthase was determined by NMR spectroscopy. The structure comprises one alpha- and two single-turn 3(10)-helices and two beta-strands, and is similar to those of the type III antifreeze proteins. Evolutionary trace analyses of the type III antifreeze protein family suggested that the class-specific residues in th...