background: hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of echinococcus granulosus. diagnosis of hydatidosis is still an unresolved problem. serological tests using crude antigens for diagnosis of e. granulosus are sensi-tive, however their specificity are not satisfactory. therefore, who recommended spe-cific serological methods using specific antigens,...

conclusions in comparison with the rate of hbcab positivity reported in other iranian studies, the rate was higher in the present study. there were a few variations, genotypes, and subtypes among the infected injecting drug users. further investigations are needed to unravel the molecular characterization of obi. results sixty-four (28%) participants were hbcab positive, 59 cases were hbcab pos...

hydatid cyst, the larval stage of cestodes echinococcus spp., is recognized as a zoonotic infection in the world. the world health organization (who) has recently classified echinococcosis in a group of neglected tropical diseases.the prevalence of echinococcus granulosus infection is high in iran due to the presence of various intermediate hosts in this country. considering the rising trend of...

conclusions although the frequency of hbsag seropositivity in the present study was close to that observed in the overall iranian population, the seroprevalence of anti-hbc was higher, possibly due to the exposure of the elderly to more risk factors. the risk factors were similar. these included a history of blood transfusions, cupping, hospital admission, endoscopy, or familial hbv/hcv infecti...

hydatid cyst is an important and zoonotic infection caused by cystic stage of echinococcus granulosus. it is a major and economic problem in most areas of the world that livestock are kept such as iran. in this study, a total of 111 buffalo,s sera from center of buffalo sperm preparation (cbsp), orumia (west azarbayjan province, iran, 2003) were examined for the presence of hydatidosis by using...

DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

background: cystic echinococcosis caused by the metacestode of echinococcus granulosus is a major problem in both humans and domestic animals health. there-fore, a standardized and approachable diagnostic tool (rapid tests) for the serodiag-nosis of cystic echinococcosis (ce) is still needed. methods: in the present work, antigen b labeled with gold nanoparticles was used to detect antibodies a...

early diagnosis of human hydatid disease by detecting the specific antibodies in patients’ sera is considered as an important step in treatment of infection. but the diagnostic efficiencies of assays greatly depend on the characteristics of antigen that is used and various conditions in performance. in present study, we tried to standardize an indirect haemagglutination test, using antigen b fo...

background: echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus echinococcus, family taeniidae . we aimed to subclone antigen b gene in pqe-30 plasmid, its expression, and purification. methods: we subcloned hi gene into pqe-30 expression vector. the recombinant vector was transformed into e. coli , m15 and mass cult...

background the transmission of hepatitis b virus (hbv) is parenteral, sexual and prenatal. prevention of vertical transmission of hbv is extremely important, because hbv infection in early life usually results in a chronic carrier state. there has been so much debate about hepatitis b surface antigen (hbsag) screening in pregnant women. objectives the aim of this study was to determine the prev...