نتایج جستجو برای: Aqueous two-phase systems (ATPS)

تعداد نتایج: 3830600  

Journal: :iranian biomedical journal 0
حمید شهباز محمدی hamid shahbaz mohammadi اسکندر امیدی نیا eskandar omidinia حشمت اله طاهرخانی heshmatollah taherkhani

background: phenylalanine dehydrogenase (phedh ec 1.4.1.20) is a nad+-dependent enzyme that performs the reversible oxidative deamination of l-phenylalanine to phenylpyruvate. it plays an important role in detection and screening of phenylketonuria (pku) diseases and production of chiral intermediates as well. the main goal of this study was to find a simple and rapid alternative method for pur...

Journal: :iranian journal of chemistry and chemical engineering (ijcce) 2008
hamid shahbaz mohammadi eskander omidinia

aqueous two-phase systems (atps) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. the aim of this work was to investigate the possibility of utilizing atps for the separation of recombinant bacillus sphaericus phenylalanine dehydrogenase (phedh). polyethylene glycol (peg) and ammonium sulfate systems were selected for our experiment. the effect of di...

Journal: :Chemical Engineering Science 2023

Liquid-liquid segmented flows in microchannels have been extensively investigated the context of nanoparticle synthesis. The enhanced mixing slugs results monodispersed particles. Earlier studies focused on Organic-Aqueous Systems (OAS). nanoparticles synthesized presence organic solutions limited applications. An alternative green route for synthesis can be developed using an Aqueous Two-Phase...

2014
Arafat M Goja Hong Yang Min Cui Charles Li

Aqueous two-phase extraction (ATPE), unique liquid-liquid extraction, involves a transfer of solute from one aqueous phase to another. ATPE includes polymer–polymer type and polymer–salt type systems for the recovery of proteins. The protein must be recovered in a highly purified form in order to improve its quality, decrease energy consumption, reduce waste and minimize costs. To acquire the h...

2016
G. Khayati

Aqueous two-phase systems (ATPSs) are composed of two immiscible aqueous phases that are in equilibrium. As one of the separation methods, aqueous two-phase extraction (ATPE) has advantages of biocompatibility, process integration capability, and large-scale feasibility1. As far as we know, ATPE has been used for the separation of proteins2, enzymes3–4, organic acids5–6, and so on. Based on the...

Journal: :journal of sciences islamic republic of iran 0
e. omidinia

phenylalanine dehydrogenase (phedh; ec 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of l-phenylalanine. the goal of this literature was to find a suitable purification method for recombinant bacillus badius phedh by practical comparison between chromatographic and polyvinyl pyrrolid...

Journal: :Polymer Chemistry 2023

Aqueous two-phase systems (ATPS) have been in the focus of polymer chemistry recent years, mainly due to their biocompatibility and various applications biomaterials biology. In present...

2011
Xin Li Zhina Lian Bo Dong Yong Xu Qiang Yong Shiyuan Yu

−Aqueous two-phase system (ATPS) was applied for extraction bioconversion of xylan by xylanase from Trichoderma viride. Phase diagrams for poly (ethylene glycol) (PEG) and sodium citrate were determined at room temperature. The ATPS composed of 12.99% (w/w) PEG6000 and 12.09% (w/w) sodium citrate was favorable for partition of xylanase and used for extraction bioconversion of xylan. Batch hydro...

E. Omidinia

Phenylalanine dehydrogenase (PheDH; EC 1.4.1.20) is an important enzyme of amino acid dehydrogenases family that increasingly used as a valuable biocatalyst in neonatal screening kits and synthesis of L-phenylalanine. The goal of this literature was to find a suitable purification method for recombinant Bacillus badius PheDH by practical comparison between chromatographic and polyvinyl pyrrolid...

Journal: :The journal of physical chemistry. B 2011
Ana Filipa M Cláudio Ana M Ferreira Shahla Shahriari Mara G Freire João A P Coutinho

In this work, the ability of Na(2)SO(4) and ionic liquids to induce the formation of acidic aqueous two-phase systems (ATPS) is investigated. Ternary phase diagrams, tie lines, and tie-line lengths for several systems were determined and reported at 298 K and atmospheric pressure. It is here shown that among the ionic liquids studied only those containing long alkyl side chains at the ions and/...

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