When uninjured cells of Valonia are placed in methylene blue dissolved in sea water it is found, after 1 to 3 hours, that at pH 5.5 practically no dye penetrates, while at pH 9.5 more enters the vacuole. As the cells become injured more dye enters at pH 5.5, as well as at pH 9.5. No dye in reduced form is found in the sap of uninjured cells exposed from 1 to 3 hours to methylene blue in sea wat...

A comparison has been made between reticulocyte preparations stained with purified azure B and with several commerically available batches of brilliant cresyl blue and new methylene blue. Marked variations were observed in the composition and staining performances of the various batches of the two commerically available dyes. Although there were no significant differences in reticulocyte counts...

Identification of positive staining is often qualitative and subjective. This is particularly troublesome in pigmented melanoma lesions, because melanin is difficult to distinguish from the brown stain resulting from immunohistochemistry (IHC) using horse radish peroxidase developed with 3,3'-Diaminobenzidine (HRP-DAB). We sought to identify and quantify positive staining, particularly in melan...

Different methods have been adopted for the removal and degradation of dyes from effluents of textile, dyeing and printing industries. These methods have their own merits and drawbacks. In the present investigation, Lead sulfide has been used as a photocatalyst for the degradation of azure-B. The effect of different parameters like the pH, concentration of dye, amount of semiconductor and light...

A new colorimetric method for determining the surface-accessible acidic lignin hydroxyl groups in lignocellulose solid fractions was developed. The method is based on selective adsorption of Azure B, a basic dye, onto acidic hydroxyl groups of lignin. Selectivity of adsorption of Azure B on lignin was demonstrated using lignin and cellulose materials as adsorbents. Adsorption isotherms of Azure...

Biodegradation of Orange II, Tropaeolin O, Congo Red, and Azure B in cultures of the white rot fungus, Phanerochaete chrysosporium, was demonstrated by decolarization of the culture medium, the extent of which was determined by monitoring the decrease in absorbance at or near the wavelength maximum for each dye. Metabolite formation was also monitored. Decolorization of these dyes was most exte...

A facile spectrophotometric method is proposed for determination of Colchicoside (COLSID) in bulk and in dosage forms using ceric ammonium sulphate (CAS). Method involves addition of a known excess of CAS to COLSID and determination of residual CAS with a fixed amount of either Azure B (AB), measuring absorbance at 633nm or Safranin O (SO), measuring absorbance at 519 nm. Calibration graphs are...

Windows Azure platform is the Microsoft implementation of cloud computing. This paper covers detailed introduction to Windows Azure Platform. Windows Azure provides resources and services for consumers. The next part describes the five main components of Windows Azure: Hardware is abstracted and exposed as compute resources. Physical storage is abstracted as storage resources and exposed throug...