Several cell surface properties of three B16 melanoma var iant lines, which exhibit differing lung-colonizing capacities, have been examined. Equal numbers of cells from the B16F10Lr and B16-F1 lines, injected i.v. into syngeneic mice, produced significantly fewer lung nodules than did cells of the B16-F10 line. These wide differences in biological behavior could not, however, be ascribed to ma...

Several cell surface properties of three B16 melanoma var iant lines, which exhibit differing lung-colonizing capacities, have been examined. Equal numbers of cells from the B16F10Lr and B16-F1 lines, injected i.v. into syngeneic mice, produced significantly fewer lung nodules than did cells of the B16-F10 line. These wide differences in biological behavior could not, however, be ascribed to ma...

BlL, a galactose-binding C-type lectin purified from Bothrops leucurus snake venom, exhibits anticancer activity. The current study was designed to elucidate the cellular mechanisms by which BlL induces melanoma cell death. The viabilities of B16-F10 melanoma cells and HaCaT keratinocytes treated with BlL were evaluated. Necrotic and apoptotic cell death, cytosolic Ca(2+) levels, mitochondrial ...

Background: Expression of the small peptide thymosin 4 is associated with angiogenesis induction, accelerated wound healing, and the metastatic potential of tumor cells. However, little is known about the mechanism(s) by which thymosin 4 promotes metastasis. Methods: Northern blot analysis and immunohistochemistry were used to examine thymosin 4 expression in mouse melanoma B16 cell lines and i...

Metastasis is a key factor that limits survival in the majority of patients with cancer. Thus, numerous efforts have been made to elucidate the molecular mechanisms involved in this phenomenon. B16‑F10 melanoma cells have been demonstrated to be highly metastatic to the lungs in mice. The aim of the current study was to investigate the role of CXC motif chemokine receptor 4 (CXCR4) in the metas...

OBJECTIVE To gain better insight into the role of glucocorticoids as modulators of cell growth, as well as to investigate the presence and characteristics of glucocorticoid receptors (GR) in mouse melanoma cells. METHODS In two different B16 mouse melanoma cell clones (B16/F10 and B16/C3) the role of synthetic glucocorticoids (triamcinolone acetonide, TA) as cell growth modulators was investi...

The role of cell surface glycoproteins in determining in vivo blood-borne arrest and survival characteristics of murine melanoma sublines of low (B16-F1) or high (B16-F10) potential to form experimental lung metastases after injection i.v. was assessed after inhibiting tumor cell protein glycosylation with tunicamycin. Incubation of B16-F1 or B16-F10 cells with 0.5 micrograms (or above) tunicam...

The correlation between the production of plasminogen activator (PA) of tumors and their metastatic potential was studied. B16 melanoma cells and "B16 mets" cells (harvested from the pulmonary metastatic nodules of C57BL/6J mice bearing B16 isografts) were examined with respect to their fibrinolytic activity (FA) in tissue culture. B16 mets cells had a significantly higher FA than did B16 cells...

The role of cell surface glycoproteins in determining in vivo blood-borne arrest and survival characteristics of murine mel anoma sublines of low (B16-F1 ) or high (B16-F10) potential to form experimental lung métastasesafter injection i.v. was as sessed after inhibiting tumor cell protein glycosylation with tunicamycin. Incubation of B16-F1 or B16-F10 cells with 0.5 /¿g(or above) tunicamycin...

Interleukin-12 (IL-12) has been used in numerous immunotherapy protocols against melanoma. However, delivery of IL-12 in the form of recombinant protein can result in severe toxicity, and gene therapy has had limited success against B16.F10 murine melanoma. The purpose of this study was to examine the effectiveness of in vivo electroporation for the delivery of plasmid DNA encoding IL-12 as an ...